Venoms: Venomous Animals and Antivenomous Serum-therapeuticsCalmette, A. (Albert)
Science
Venoms: Venomous Animals and Antivenomous Serum-therapeutics
Calmette, A. (Albert)
Antitoxins; Poisonous snakes; Toxins; Venom
[Illustration: FIG. 87.--CHLOROFORMING A COBRA IN ORDER TO
COLLECT VENOM, AT THE FRENCH SETTLEMENT OF PONDICHERRY, IN INDIA
(STAGE II.).]
Except when a snake is _moulting_, the venom can be extracted from its
glands about every fortnight; and it is better that the extraction be
not performed concurrently with artificial feeding, since, owing to the
fact that the venom serves the animal as digestive juice, the reptile
will soon perish if deprived of the means of digesting the food that it
is obliged to receive. It is best, therefore, to select one day of the
week for artificial feeding, and the corresponding day of the following
week for the extraction of the venom.
When the venom has been collected, it must immediately be in a
desiccator over calcium chloride or sulphuric acid, in order to dry
it rapidly. In hot countries, and where no laboratory specially
equipped for the purpose exists, it will suffice to dry the venom in
a current of air, or even in the sun. It then concretes in scales of
a citrin colour, more or less dark, according to the concentration
of the liquid. In this dry condition, placed in well-corked bottles,
protected from damp air, it may be kept almost indefinitely without
losing anything of its original toxic power. On the contrary, if the
desiccation be imperfect it undergoes a somewhat rapid change, and
assumes a disagreeable odour of meat peptone. I have kept samples of
various venoms, dried as described, for _fifteen_ years without any
sensible diminution of their activity.
[Illustration: FIG. 88.--COLLECTING COBRA VENOM AT PONDICHERRY
(STAGE III.).]
CHAPTER V.
_THE CHEMICAL STUDY OF SNAKE-VENOMS._
In the condition in which they are received on issuing from the
glands, venoms always present the appearance of a thick saliva, of
an oily consistency and more or less tinged with yellow, according
to the species of snake by which the poison has been produced. They
are entirely soluble in water, the addition of which renders them
opalescent. Tested with litmus they exhibit a slightly acid reaction;
this acidity, which is due to the presence of a very small quantity
of an indeterminate volatile acid, disappears on desiccation, so that
solutions of dried venom are neutral. The taste of venoms is very
bitter. Their density, which is slightly greater than that of water,
varies from 1030 to 1050.
Venoms are composed of a mixture, in variable proportions, of proteid
substances, mucus and epithelial _débris_, fatty matters and salts
(chlorides and phosphates of lime, ammonia and magnesia), with from 65
to 80 per cent. of water.
The elementary analysis of Cobra-venom made by H. Armstrong[7] gave the
following results:--
Carbon 43·04 per cent.
Hydrogen 7·00 “
Nitrogen 12·45 “
Sulphur 2·50 “
Residue Small quantities.
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