One of the most noted practitioners in Southern Italy, after the death of Trotula and her compeers, was one Margarita, who had studied medicine in Salerno. One of her patients was no less a personage than Ladislaus, King of Naples. Among those that had diplomas for the practice of surgery were Maria Incarnata, of Naples, and Thomasia de Matteo, of Castro Isiae. That women enjoyed in Rome the same privileges in the practice of medicine and surgery as their sisters in the southern part of the peninsula is manifest from an edict issued by Pope Sixtus IV in confirmation of a law promulgated by the Medical Faculty of Rome, which reads as follows: "No man or woman, whether Christian or Jew, unless he be a master or a licentiate in medicine, shall presume to treat the human body either as a physician or as a surgeon."[207] In central and northern Italy--in Florence, Turin, Padua, Venice--as well as in the southern part, we find constantly recurring instances of women practicing medicine and surgery and winning for themselves an enviable reputation as successful practitioners. But after the decline of Salerno, consequent on the establishment by Frederick II of a school of medicine in Naples, the great center of medicine and surgery, as of civil and canon law, was Bologna.[208] So renowned did it become as a teaching and intellectual center that it was, as Sarti informs us, known throughout Europe as _Civitas Docta_--the learned city--and _Mater Studiorum_--the mother of studies. On its coins were stamped the words _Bononia Docet_--Bologna teaches--and on the city seal, which is still used for certain public documents, were the words _Legum Bononia Mater_--Bologna, the Mother of Laws.