A bacteriological study of ham souringMcBryde, Charles Neil
Science
A bacteriological study of ham souring
McBryde, Charles Neil
Ham -- Microbiology
The ordinary pickle bacteria, therefore, would
not multiply as rapidly in the interior of the hams and would not find
their way into the bone marrow as soon as would a strictly anaerobic
organism.
Pure cultures of the sour-ham bacillus, recovered from the meat and
bone marrow of the injected hams, were compared with cultures of the
original bacillus used for inoculating the hams, and were found to be
identical. Furthermore, the bacillus with which the hams were injected
was recovered from the injected hams at points far removed from the
original point of injection, showing that the organism had multiplied
and extended throughout the bodies of the hams and that it was clearly
responsible for the souring which the hams had undergone.
Sound hams from tierces 2 and 4 were examined bacteriologically in the
same manner as the injected hams, and some of the cultures showed the
ordinary pickle bacteria, but in not a single instance did egg-pork
cultures yield a sour odor, and in no case could the sour-ham bacillus
be demonstrated in any of these hams.
Microscopic sections and teased preparations of the muscle fibers in
salt solution were prepared from several of the sour hams in this
experiment, and these preparations showed the same histological changes
and the same distribution of bacilli as noted in the natural sours.
In Plate III, figures 1 and 2, sections are shown of artificially
soured hams, that is, hams which were artificially soured by injections
of culture; and if these figures be compared with the sections made
from hams which had undergone spontaneous souring (see Pl. II, figs. 1
and 2) the similarity in the form and distribution of the bacilli will
be at once apparent.
[Illustration:
BUL. 132, BUREAU OF ANIMAL INDUSTRY, U. S. DEPT. OF AGRICULTURE.
PLATE III.
FIG. 1.—SECTION THROUGH MUSCULAR TISSUE OF ARTIFICIALLY SOURED
HAM, SHOWING DISTRIBUTION OF BACILLI BETWEEN THE MUSCLE FIBERS,
WHICH ARE SHOWN IN CROSS SECTION. THE DARK LINES AND MASSES
BETWEEN THE MUSCLE FIBERS REPRESENT CLUMPS OF BACILLI.
(Pen-and-ink drawing made with camera lucida from section stained by
the Gram-Weigert method to show bacteria. × 85.)]
FIG. 2.—SECTION THROUGH MUSCULAR TISSUE OF ARTIFICIALLY SOURED
HAM, SHOWING INDIVIDUAL BACILLI BETWEEN THE MUSCLE FIBERS,
WHICH ARE CUT LONGITUDINALLY.
(Pen-and-ink drawing made with camera lucida from section stained by
the Gram-Weigert method to show bacteria. × 320.)]
Public-domain text, read in full here on John Shaqi.
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