_Tierce 2._—Contained 20 hams which were pumped in the same manner as those in tierce 1. The curing pickle was the same as that used for tierce 1, but without the addition of culture. This tierce was put down as a check on tierce 1, the hams being cured under exactly the same conditions, but without the addition of culture to the curing pickle. Result: One of the hams which was pumped in the shank only developed slight souring in the body. The remainder of the hams were sweet. Comparing tierce 1, which contained the inoculated pickle, with tierce 2, the check tierce which contained uninoculated pickle, we find there was practically no difference in the final result. In tierce 1 two of the hams developed slight souring, while in tierce 2 one of the hams became slightly sour. All of these hams had been pumped in the shank only. The fact that one of the hams in the check tierce developed slight souring was undoubtedly due to bacterial contamination in pumping or in the handling which the hams underwent prior to pickling, and the slight souring of the two hams in tierce 1 must also be attributed to the same cause or causes, for had the souring in these last hams resulted from the penetration of the bacteria from the pickling solution a higher percentage should have become sour. Furthermore, if the souring of the two hams in tierce 1 had resulted from the penetration of the bacteria from the curing pickle, the souring should have been general throughout the bodies of these hams, whereas the souring was only evident around the bone and was slight in degree. From this experiment the conclusion would seem justified that the bacillus which causes ham souring does not usually find its way into the bodies of the hams from the curing pickle, although it would be going too far, perhaps, to say that infection never takes place from the curing pickle. The experiment, however, indicates clearly that the curing pickles are certainly not the main channel through which the hams become infected. In referring to the curing pickles, it should be understood that we refer here to the pickling solutions in which the hams are immersed, and not to the pumping pickles. The possibility of infection through the pumping pickle will be discussed later. POSSIBLE INFECTION THROUGH MANIPULATION OR HANDLING. There are at least three possible ways in which hams may become infected from the handling which they receive in preparation for, or during the process of curing, viz: From the thermometers used in taking the inside temperatures of the hams, from the pumping needles, and from the billhooks used in lifting the hams. INFECTION FROM HAM THERMOMETERS.