When the reaction of the agar is neutral or slightly alkaline, extensive gas formation occurs and the agar is often much broken up. The cultures developed a disagreeable, somewhat putrefactive odor, but did not give the characteristic sour-ham odor obtained from the egg-pork cultures. The organism was also grown on anaerobic agar plates by Zinsser’s method, which is said to give absolutely anaerobic conditions. The colonies on agar have a cottony or woolly appearance at first, and spread slowly, with slightly irregular margins. In glucose-pork-agar to which azolitmin was added the azolitmin in the lower portion of deep stab cultures was completely decolorized in five days at room temperature (20° to 25° C). In glucose-pork-agar containing neutral red the red color in the lower portion of the tube was changed to yellow with the development of fluorescence. _Neutral gelatin._—Tubes of ordinary neutral gelatin without the addition of glucose were inoculated and held at ice-box temperature (8° to 10° C). At five days a delicate white growth appeared along the line of stab in the lower portion of the tube. At seven days the growth showed fine radial striæ, presenting an arborescent or tree-like appearance, and extended halfway from the line of stab to the walls of the test tube. At two weeks the growth had caused a delicate clouding of the medium in the lower portion of the tube. At three weeks the gelatin in the lower portion of the tube had become liquefied and the growth had settled to the bottom as a white precipitate. In gelatin containing glucose, gas bubbles are formed in the depth of the medium through the splitting up of the glucose, and the characteristic arborescent growth is obscured. _Glucose-pork-bouillon._—This medium was prepared from pork instead of beef and contained 1 per cent of glucose. The best results were obtained when the reaction of the medium was neutral or slightly alkaline. Culture tubes, which had been previously boiled to expel the contained air and then inoculated, were held in a Novy jar, in an atmosphere of hydrogen at a temperature of 20° to 25° C. At three days the tubes showed well-marked clouding. At one week the growth appeared as a heavy, white, flocculent, cottony precipitate in the bottom of the tubes with a slight flocculent precipitate above. When the culture was removed from the jar and shaken, the heavy, flocculent precipitate at the bottom of the tube broke up without much difficulty, giving rise to a heavy uniform clouding with some small floating masses, which soon settled to the bottom. On shaking the tube some evolution of gas in the form of very fine bubbles was noticed.