The spiny dogfish, which is the particular subject of this guide, is the species most frequently supplied to laboratories. However, the spiny and smooth dogfishes are so much alike that the latter may be easily dissected with these directions. Where marked differences between the forms exist the structure of Eugaleus is described separately. The student of anatomy should realize that dissection is for the purpose of enabling him to see for himself the structures which exist, and that no dissection is satisfactory until the anatomical arrangements mentioned in the text can be completely demonstrated in his specimen. The importance of knowing the structure of the elasmobranch is so great in comparative anatomy and embryology that it is worth while to make a thorough dissection of one of this class. As the dogfish is frequently the first major vertebrate form to be studied in detail, these directions have been written to conform to the needs of the student who is beginning comparative anatomy. The arrangement of sections in this guide is intended to permit the omission of some which it may not be considered desirable to include in the work of a class. EXTERNAL CHARACTERS The spindle-shaped body tapers from near the middle toward both head and tail; the head is flattened on both the dorsal and ventral sides, while the remainder of the body is nearly round, with a lateral compression which is not pronounced except in the caudal portion. The general color of the back and sides is gray; darkest above, where the skin is spotted with scattered, small, round, light spots. The color of the upper parts shades into the yellow white of the ventral surface. Can head, trunk, and tail regions be distinguished? If so, what characters determine the extent of each? A little above the middle of the side of the body is the _lateral line_, (distinguished partly by color, partly by being slightly elevated), which extends from the back of the head to the tail. Cut through the skin across the lateral line at several points along the body and notice the canal which lies in the dermis under the lateral line. This is the _lateral line canal_, which opens to the surface by numerous pores (too minute to be seen), and contains a series of special sense organs along its dorsal and inner surfaces. Near the base of the caudal fin the lateral line canal passes into a groove which continues the lateral line to within a short distance of the edge of the fin. The lateral line canal in its development begins as a groove along the side of the body which becomes closed by the fusion of its edge except in this terminal portion. The open groove does not appear in Eugaleus.