A Guide for the Dissection of the Dogfish (Squalus Acanthias)Griffin, Lawrence Edmonds
Science
A Guide for the Dissection of the Dogfish (Squalus Acanthias)
Griffin, Lawrence Edmonds
Dogfish; Fishes -- Anatomy
Only the head and anterior part of the trunk will be required for the
dissection of the nervous system. Cut across the body back of the
pectoral fins; the posterior part of the body will not be required
further unless it is desired to study the muscles and skeleton.
The manner of dissecting the brain depends somewhat upon the specimens
at the disposal of the student. If a large head is to be used especially
for the dissection of the cranial nerves, only the brain, eye and ear
need be studied in the present specimen. But in most cases it will be
found best for the student to dissect the first dogfish as thoroughly as
possible, working out the cranial nerves as well as the brain, and
reserving the second head for a thorough review of the entire nervous
system. Chapter III of Herrick and Crosby’s “Laboratory Outline of
Neurology” should be used in connection with such a review.
If a line be drawn over the dorsal surface of the head connecting the
two spiracles, two small pores will be found near the middle. These are
the external apertures of the ducti lymphatici. Cut carefully through
the skin in a small circle around the pores, and remove the skin from
the remainder of the dorsal surface of the skull without disturbing the
small section containing the pores. The latter part should now be lifted
gently; beneath it will be seen two delicate tubes passing from the
pores to apertures in a depression of the skull below them. These tubes
are the _ducti endolymphatici_, through which a passage exists between
the internal ear and the exterior. As they cannot be preserved in the
subsequent dissection, the pores by which they pass through the skull to
the internal ear should be found now, and a memorandum-sketch made of
the ducts themselves.
DORSAL SURFACE OF THE BRAIN. The roof of the skull should be removed
from over the brain. Use a sharp scalpel and take very thin slices of
cartilage. Do not cut beyond the brain at the sides. No attempt should
be made at this time to expose more than the dorsal surface of the
brain.
Above the anterior end of the brain there is a small median foramen
through the skull, the _epiphysial foramen_. A strand of tissue, the
_epiphysis_, leading from this to the surface of the brain, should be
carefully observed and retained. The cartilage should also be cut away
from above the portion of the spinal cord next the skull. Gently wash
away any coagulated lymph.
The brain and spinal cord are invested by two membranes (_meninges_).
The tough _dura mater_ lines the cavity in which they lie, clinging
closely to the cartilage; in fact it forms the perichondrium of the
internal surface of the cranium. The _pia mater_ envelops closely the
brain and cord, and contains numerous blood vessels. Between the two is
the _arachnoid space_, traversed by occasional fine threads of
connective tissue and filled with lymph.
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