A Guide for the Dissection of the Dogfish (Squalus Acanthias)Griffin, Lawrence Edmonds
Science
A Guide for the Dissection of the Dogfish (Squalus Acanthias)
Griffin, Lawrence Edmonds
Dogfish; Fishes -- Anatomy
The optocoels are large cavities within the optic lobes which open into
the aqueduct of Sylvius.
A large metacoel in the metencephalon opens into the myelocoel. The
myelocoel is also continued into the corpora restiforma; posteriorly it
joins the _central canal_ which extends down the center of the spinal
cord.
DISSECTION OF THE EYE. Remove one of the eyes from its orbit, and divide
it into inner and outer halves by an equatorial cut around the eyeball
(not directly through it, as this tears the lens from its fastenings).
Place the halves under water and observe:
In the inner half:
The _posterior chamber_, the cavity of the eyeball which has been
opened. During life it is filled by a gelatinous substance, the
_vitreous humor_.
The _retina_, a delicate yellowish-white membrane lining the interior of
the eye, loosely attached to the outer coats except at the point of
entrance of the optic nerve.
The _choroid coat_, a thin, black membrane outside the retina. It can be
pulled away from the outer coat quite easily except near the optic
nerve.
The _sclerotic coat_, the outer coat of the eye. This is composed of
connective tissue having an almost cartilaginous consistency, is only
slightly pigmented, and is somewhat translucent. The muscles of the eye
are inserted upon the sclerotic.
In the outer half:
The _ora serrata_, an irregular line along which the retina ends.
The _iris_, a fold of the choroid extending inward like a shelf, and
perforated centrally to form the pupil. Around the iris the choroid is
folded radially into the _ciliary processes_.
The _lens_, a spherical body, transparent and elastic during life, but
opaque and hard in preserved specimens. It projects into the pupil and
is suspended from the ciliary processes by a delicate membrane, the
_suspensory ligament_.
The _anterior chamber_, in front of the iris and lens, filled with a
watery fluid, the _aqueous humor_.
The transparent _cornea_, forming the outer side of the eyeball,
continuous with the sclerotic.
Take out the other eye and cut it in two by a section through the pupil
and optic nerve. Review the relation of the parts.
THE SKELETON
There seems to be no easy way of cleaning the skeleton of dogfish which
have been preserved in formalin or alcohol, the only procedure being to
cut, pick, and scrape the flesh away from the skeleton. Time and
patience are required, but if these are allowed there is no reason why
all the parts of the skeleton cannot be thoroughly studied. Specimens
which have been preserved in brine are more easily skeletonized.
The skeleton is entirely composed of cartilage which, in large species
of elasmobranchs and in old individuals of small species, becomes
impregnated with lime salts, in some cases to such an extent as to
resemble soft bone.
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