First Sunday at Sea.—Gulf Stream.—Gulf-Weed.—Lectures proposed.—First Lecture: On the Gulf Stream in the Gulf Stream.—Aquarium established on board.—Second Lecture.—Rough Sea.—Peculiar Tint of Water.—Third Lecture: Laying out Work of Expedition in Brazil; Distribution of Fishes in Brazilian Rivers; its Bearing on Origin of Species; Collecting of Eggs.—Tropical Sunset.—Fourth Lecture: Plan of Geological Investigations with special reference to Glacial Phenomena in South America.—Flying-Fish.—Fifth Lecture: Glacial Phenomena, continued.—Second Sunday at Sea.—Rough Water.—Sixth Lecture: Embryological Investigations as a Guide to sound Classification.—Seventh Lecture.—Moonlight Nights.—Trade-Winds.—Eighth Lecture: Importance of Precision in Localizing Specimens.—Southern Cross.—Ninth Lecture: Fresh-water Fishes of Brazil.—Easter Sunday.—First Sight of South American Shore.—Olinda.—Pernambuco.—Catamarans.—Tenth Lecture: Methods of Collecting.—Eleventh Lecture: Classification of Fishes as illustrated by Embryology.—Preparations for Arrival.—Twelfth Lecture: Practical Lesson in Embryology.—Closing Lecture: Transmutation Theory; Intellectual and Political Independence.—Resolutions and Speeches.—Singular Red Patches on the Surface of the Sea 1–45 CHAPTER II. RIO DE JANEIRO AND ITS ENVIRONS.—JUIZ DE FORA. Arrival.—Aspect of Harbor and City.—Custom-House.—First Glimpse of Brazilian Life.—Negro Dance.—Effect of Emancipation in United States upon Slavery in Brazil.—First Aspect of Rio de Janeiro on Land.—Picturesque Street Groups.—Eclipse of the Sun.—At Home in Rio.—Larangeiras.—Passeio Publico.—Excursion on the Dom Pedro Railroad.—Visit of the Emperor to the Colorado.—Cordiality of the Government to the Expedition.—Laboratory.—Botanical Garden.—Alley of Palms.—Excursion to the Corcovado.—Juiz de Fora Road.—Petropolis.—Tropical Vegetation.—Ride from Petropolis to Juiz de Fora.—Visit to Senhor Lage.—Excursion to the Forest of the Empress.—Visit to Mr. Halfeld.—Return to Rio.—News of the Great Northern Victories, and of the President’s Assassination 46–79 CHAPTER III. LIFE IN RIO CONTINUED.—FAZENDA LIFE.