The antiseptic fluid having been prepared in a convenient vessel, the syringe, after having been carefully examined and found in good working order, should be slowly filled, adjusted to the stop-cock joint, the cock opened, and the syringe slowly discharged. Shutting off the cock, to prevent escape of the fluid, the syringe may now be detached, refilled and again discharged, the process being repeated until the vessels are filled with the fluid, which may be known from the resistance offered to its introduction. After a few syringefuls have been thrown into the vessels, the blood will be seen flowing more or less freely from the jugular, and which will gradually change to the clear antiseptic fluid; then the vessel may be tied, to prevent any further loss. To facilitate the flow of the fluid into the vessels of the extremities, free flexion and extension of all the limbs should be made; and after some minutes, another effort made to force in more of the fluid. A successful filling of all the vessels may be known by the distension of the superficial veins of the chest and arms, accompanied with an escape of a dark fluid from the nostrils and, perhaps, mouth, from an oozing of the fluid through the capillary vessels of the mucous membranes of the head. If the extemporized gum syringe be used, its pipe should be tied in the vessel as in the other case, the opposite end of the tube placed in the vessel of fluid, and the same thrown in by pressure of the ball. The operation being completed, a ligature should be tightly placed around the artery below the pipe, the latter removed and the incision sewed up. _Changes Resulting from the Operation._ As the vessels become filled during the operation, the abdomen will gradually swell, the chest become more round and full, the face and eyelids become puffy, while the superficial veins will appear full and dark from the contained blood. In a few hours the skin assumes a pale, ashen hue, with a parchmentlike feel, which will be a sure indication of the success of the operation. In a few days the puffed appearance of the face will have disappeared, when no further change will be noticed, until after several weeks the ears, with the tip of the nose, and ends of the fingers, begin to shrivel and dry up. CHAPTER VII. PRESERVATION OF SPECIMENS OF MORBID ANATOMY. Many valuable and interesting pathological specimens are continually being lost, from a want of knowledge, or a careless neglect on the part of the physician. That the fullest benefit may result from a post-mortem examination, any rare or interesting specimen should be carefully preserved. Should the physician feel no interest in forming a cabinet of morbid anatomy for himself, he should then deposit the specimen in the anatomical museum of some medical school, where it may be permanently preserved, and the profession thus benefited.