Alcoholic Fermentation: Second Edition, 1914Harden, Arthur
Science
Alcoholic Fermentation: Second Edition, 1914
Harden, Arthur
Fermentation
The origin of putrefaction was definitely attributed by Schwann to the
presence of living germs in the air, and the similarity of the result
obtained with yeast suggested the idea that alcoholic fermentation was
also brought about by a living organism, a conception which was at
once confirmed by a microscopical examination of a fermenting liquid.
The phenomena observed under the microscope were similar to those
noted by Cagniard-Latour, and in accordance with these observations
alcoholic fermentation was attributed to the development of a living
organism, the fermentative function of which was found to be destroyed
by potassium arsenite and not by extract of Nux vomica, so that the
organism was regarded rather as of vegetable than of animal nature.
This plant received the name of "Zuckerpilz" or sugar fungus (which
has been perpetuated in the generic term Saccharomyces). Alcoholic
fermentation was explained as "the decomposition brought about by
this sugar fungus removing from the sugar and a nitrogenous substance
the materials necessary for its growth and nourishment, whilst the
remaining elements of these compounds, which were not taken up by the
plant, combined chiefly to form alcohol".
Kützing's memoir, the third of the trio [1837], also dates from
1837, and his opinions, like those of Cagniard-Latour, are founded
on microscopical observations. He recognises yeast as a vegetable
organism and accurately describes its appearance. Alcoholic
fermentation depends on the formation of yeast, which is produced
when the necessary elements and the proper conditions are present
and then propagates itself. The action on the liquid thus increases
and the constituents not required to form the organism combine to
form unorganised substances, the carbonic acid and alcohol. "It is
obvious," says Kützing, in a passage which roused the sarcasm of
Berzelius, "that chemists must now strike yeast off the roll of
chemical compounds, since it is not a compound but an organised body,
an organism."
These three papers, which were published almost simultaneously,
were received at first with incredulity. Berzelius, at that time
the arbiter and dictator of the chemical world, reviewed them all
in his "Jahresbericht" for 1839 [1839] with impartial scorn. The
microscopical evidence was denied all value, and yeast was no more
to be regarded as an organism than was a precipitate of alumina.
Schwann's experiment (p. 6) was criticised on the ground that the
fermenting power of the added yeast had been only partially destroyed
in the [p008] flasks in which fermentation ensued, completely in
those which remained unchanged, the admission of heated or unheated
air being indifferent, a criticism to some extent justified by
Schwann's statement, already quoted, of the uncertain result of the
experiment.
Public-domain text, read in full here on John Shaqi.
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