In the first place the property of carrying particles toward the anterior end of amebas does not appear to be of any advantage. That is, whatever the movements of the outer layer may be, the ameba does not appear to be better off when particles are carried forward than when none are carried, for such particles are very small and almost without exception devoid of food value. The particles are masses of debris which accidentally adhere to the ameba, and the ameba makes no visible effort to make such particles adhere, nor to get rid of them. The ameba seems to be quite indifferent to the presence of such particles. On the other hand, as Schaeffer (’17) has pointed out, the capacity for transporting particles cannot but be looked upon as a hindrance to locomotion. As has been stated, the surface film moves in the same direction as the ameba. Whenever the surface film comes against a solid object, it pushes against the object, and nullifies to a certain, though small, extent the energy expended in moving forward. And it will be seen without further argument, of course, that the energy involved in carrying particles forward is not only itself lost but consumes an appreciable part of the energy available for forward movement. This fact, together with the universal occurrence of this phenomenon among amebas indicates beyond question that it is intimately associated with ameboid movement as it is ordinarily understood in amebas, and that it is almost certainly a “necessary” physical consequence of the more fundamental physical processes involved in the movement of amebas. That the third layer moves in the same general direction as the ameba has already been mentioned. The direction of a moving particle is however not necessarily parallel with the stream of endoplasm below. In a retracting pseudopod that lies nearly parallel to and by the side of the main advancing pseudopod, the particles on the far side and near the base frequently move across the pseudopod at an angle (and therefore also across the endoplasmic stream), and up the active pseudopod on the near side. This shows conclusively that the direction of flowing endoplasm by itself has no direct connection with the direction of flow of the surface layer.