VI. But that the series of experiments I had undertaken might be better calculated to establish the theory of animal electricity, and as it was difficult to find any of the solid metals homogeneous throughout, I had recourse to mercury, which, by the help of chemistry, may be brought to a very considerable degree of purity. For the greater convenience in the employment of this substance, I invented the following apparatus:—Two glass vessels are so arranged (Plate IV. fig. 1.) that the one stands above the other: the upper one is filled with mercury, and the spinal marrow of a prepared frog is placed in it; and by means of a hole in the bottom of it, which may be opened at pleasure, the mercury can be made to fall on any part of the muscles of the same animal placed in the lower vessel. The stream of mercury occasions convulsive movements to take place in the muscles; and yet in this case the mercury forms both the armature and the arc: consequently the electricity in both is the same, and can exercise no action. When contractions therefore take place, they cannot be ascribed to the electricity of the metal. Should it be said that the mercury as it runs down may excite electricity from the sides of the glass vessel, as is the case with the mercury in the upper part of a barometer, which, when in the least agitated, shines with an electric light, any doubt on this subject may be easily removed by substituting vessels of wood instead of those of glass. VII. When these vessels are used, care must be taken to pay attention to one circumstance, which may prevent the success of the experiment. As the spinal marrow is exceedingly light, it will float on the surface of the mercury; whereas it ought to be immersed in that metal. It will be necessary therefore to press down the spinal marrow below the surface of the mercury, by means of a glass rod, or any other non-conducting body, so that it may be entirely immersed; otherwise some irregularity may take place, and the experiment fail. To perform it with the greatest convenience, provide a glass vessel consisting of two branches in the form of a syphon, as represented in Plate IV. fig. 2., one of which is wide, and has its edges reflected inwards, so as to form an inverted cone, ending in an aperture that can be opened and shut at pleasure. If mercury be poured into the narrower branch, so as to fill the interior part of the vessel around the inverted cone, it will not be able to rise up into the latter, until the aperture in its apex be opened. When this arrangement has been made, immerse the spinal marrow of a frog in the mercury contained in the smaller branch of the vessel, and place the muscles in the conical part, which is empty: if the aperture be then opened, the mercury, endeavouring to bring itself into a state of equilibrium, will come in contact with the muscles, and contractions will be produced; as an arc of quicksilver will thus be speedily formed between the nerves and the muscles.