Several philosophers have endeavoured to obtain this interesting result. Professor Volta, in a letter which he addressed to me, in Brugnatelli’s Journal, observed, “that various parts of animals can excite Galvanism, independently of metals.” Galvani, a short time before his death, proposed two ingenious methods of obtaining this result, and gave me a description of them. This, however, has not been able to destroy the incredulity of some philosophers, who hitherto have confounded Galvanism with metallic electricity, under an idea that all contractions proceed from irritation, produced by the action of metals. For this reason I have, with confidence, announced my method, which enables any one to observe this important result. EXPERIMENT I. Having prepared a frog in the usual manner, I hold the spinal marrow in one hand (Plate I. fig. 3.), and with the other form an angle with the leg and foot, in such a manner that the muscles of the leg touch the crural nerves. On this contact strong contractions, forming a real electrico-animal alarum (_carillon_), which continue longer or shorter according to the degree of vitality, are produced in the extremity left to itself. In this experiment, as well as in the following, it is necessary that the frogs should be strong and full of vitality, and that the muscles should not be overcharged with blood. EXPERIMENT II. By observing the directions already given, very strong convulsions will be obtained; but they must not be ascribed to the impulse produced by bringing the nerve into contact with the muscle. If the experiment be repeated, covering the muscle, at the place of contact, with a non-conducting substance, the contractions will entirely cease; but they will be re-produced as soon as the nerve is made to touch the muscular substance. In performing this experiment, in public, I obtained several times more than two hundred successive contractions; but this was never the case when I formed the same contact with the muscle by means of a conducting substance, and even with a plate of metal.