An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
A very simple and striking method of determining whether any granule is
composed of starch or not, consists in adding to it, when placed in
water on a slide, a drop of solution of iodine. As soon as this touches
the granule, it assumes a beautiful purple colour, the depth of tint
depending upon the quantity of the iodine-solution; if this be very
considerable, the granule appears almost black. The section of potato
forms a very interesting object when moistened with the iodine-solution,
the starch-granules becoming beautifully coloured, whilst the cell-wall
remains colourless, and the protoplasm becomes yellow.
The form of the starch-granules differs in different plants, so that
the kind of plant from which starch has been derived may be
distinguished by attention to the size, form, and structure of its
starch-granules. Thus, the granules represented in Pl. I. fig. 3, which
it will be noticed are all drawn under the same power, are derived from
different plants,--_a_ being those of wheat-flour, in which the hilum is
obscure, and the rings faint; _b_ is a granule of West Indian arrowroot,
in which the hilum forms a transverse crack; _c_ is a granule of
potato-starch, in which the hilum is a dot, and the rings are very
distinct; _d_ represents the compound granules of the oat, the separate
granules being figured below; _e_ is a granule of lentil-starch, with
its long dark hilum and elegant oval concentric rings; and _f_
represents a compound and separate granule of rice-starch. It will be
noticed that the granules of oat-and rice-starch are angular, as it is
called.
The knowledge of the peculiar forms of the starch-granules is important
in a practical point of view, for it enables us to recognize them when
mixed as an adulteration with other substances, and also to distinguish
the different kinds of starch from each other. Thus table-mustard, as it
is called, is principally composed of the cheaper wheat-or pea-flour,
which is easily recognized by the structure of the starch-grains.
Arrowroot is considerably dearer than potato-starch; hence in trade the
latter is fraudulently sold for the former, the adulteration being
detected with difficulty by the eye, but easily under the microscope.
Again, rice is largely mixed with wheat-flour, as it makes inferior
flour into very white bread; and this may also be readily detected under
the microscope. The reader can now understand how valuable the
microscope is in detecting adulterations, with a knowledge of the
various forms and structures of substances, especially with the aid of a
few chemical tests.
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