The post-mortem examination revealed the presence of ulcers in the stomach and duodenum. Some of the ulcers had nearly perforated the walls of the stomach and duodenum. The heart was in diastole; brain and myel appeared normal. As might be expected from the emaciated condition, the entire body was anæmic. In a second case 60 to 70 cubic centimeters of a more concentrated decoction were fed daily, with other food as before, to a vigorous adult cat. The symptoms of inactivity, loss of appetite, rough coat, diarrhea, and the peculiar expression of countenance were as in the first case. By the twelfth day the cat was wasted almost to a skeleton, and was correspondingly weak. Paralysis of the hind limbs came on, and the cat died on the thirteenth day. There were no periods of excitement in this case. These cats developed a craving for the decoction and "would beg for it as an ordinary kitten does for milk, and when supplied would lie down contented." Doctor Day made controls with healthy animals under the same conditions, with the exception that they received no loco plant. She also fed a young wild jack rabbit on milk and grass for a few days and then substituted fresh loco plants for grass. At first the "loco" was refused, but after two or three days the "loco" was eaten with as much relish as the grass had been. After ten days of the milk and "loco" diet the rabbit was found dead, with the head thrown back and the stomach ruptured. Subcutaneous injections of the concentrated decoction caused nervous twitchings in frogs and kittens, and if large amounts were used death followed in from one to two hours from paralysis of the heart. The same symptoms were produced in frogs by the injection of an alcoholic extract of the residue left after the evaporation to dryness of the decoction. In other words, Doctor Day was able to produce a chronic form of loco poisoning with the characteristic symptoms so often described save in the occurrence of diarrhea. Diarrhea is not usually noted on the range. Sayre had already reported an ulcerated condition of the intestines of a locoed cow similar to that described by Doctor Day as occurring in cats. Doctor Day urged that the reason previous experimenters failed to produce symptoms was that they had used too small an amount of the plant and that by systematic feeding to healthy cats cases of loco disease may be produced. Storke states that "Dr. V. C. Vaughan, of the University of Michigan, has since fully corroborated Dr. Day's views."[104] In her experiments Doctor Day used the leaves, roots, and stems of the plants gathered in September. She believed that the greatest amount of poison is present in autumn and winter. She later undertook the isolation of the active principle, and proceeded as follows:[105]