On October 24, 1907, a rabbit weighing 1,346.5 grams was fed with a solution of 25 milligrams of crystallized barium acetate. On the next day the weight was 1,318 grams, and the dose was repeated. On October 26 it weighed 1,275.7 grams, and the dose was repeated; on October 30 it weighed 1,332 grams, and on October 31 its weight was 1,375 grams. The animal died at night on November 6; weight, 1,134 grams. The post-mortem examination, made with Dr. Meade Bolton, of the Bureau of Animal Industry, was negative save for the presence of necrotic tissue in one enlarged thyroid. On October 24, 1907, a rabbit weighing 1,332 grams was fed with a solution of 25 milligrams of crystallized barium acetate. On the next day the animal weighed the same, and the dose was repeated. On October 26 it weighed 1,289 grams, and the same amount of barium was given. On October 28 the weight was 1,219 grams and two days later 1,289 grams. On October 31, 1907, a rabbit weighing 723 grams was fed with a solution of 25 milligrams of barium acetate. This rabbit was fed in all nine times during a period of ten days. At the end of this time it weighed 779 grams and died six days later, weighing 723 grams. The post-mortem was negative. A rabbit weighing 779 grams was also fed on October 31, 1907, with a similar amount of barium. This dose was repeated six times during an interval of eight days. At the end of that time the animal still retained its normal weight. On November 14, 1907, it weighed 709 grams, having lost 70 grams. Thus after daily doses of 0.1 gram of crystallized barium acetate no symptoms appeared until the fifth day, when death resulted. After the similar administration of 50 milligrams severe symptoms developed on the same day, but the animal recovered. After the administration of 25 milligrams on three successive days the animal died. In other cases of feeding 25 milligrams for several successive days, some lost weight and died; others merely lost in weight, but recovered. Bary fed a rabbit weighing 0.9 kilogram a solution of 30 milligrams of barium chlorid on one day, on the second day 90 milligrams, and on the third day 30 milligrams. The only symptom noted was diarrhea. The animal died on the fifth day. In other words, after feeding small doses of barium salts for several days acute symptoms suddenly set in, showing a cumulative action. This cumulative action has been noted on man.[193] Onsum[194] fed a medium-sized rabbit daily with small doses of barium carbonate, beginning with 20 milligrams. When the total amount reached 0.19 grams the rabbit died. The animal before death showed paralysis, respiratory disturbances, and fall in temperature. The sensibility of the cornea diminished, but the pupils responded to light. The stomach walls showed ecchymoses and the blood vessels of the brain, the spinal cord, and the abdominal vessels were dilated. Emboli in the pulmonary arteries were also noted.