=APPLICATION OF THE RESULTS OF THESE INVESTIGATIONS TO THE RANGE.= It has been calculated that a medium estimate of food for cattle on green fodder is about 60 pounds (30 kilos) a day.[250] Calculating this entirely in terms of _Aragallus lamberti_ and allowing 10 per cent of moisture for these plants (Sayre) would make 27 kilos of dry loco eaten by each animal per diem. In the analysis of the writer of one _Aragallus lamberti_ from Hugo, Colo., it was found to yield 12.44 per cent of ash, and the barium content corresponded to 2.6 milligrams BaSO_{4} in each gram of the ash. This would correspond to 10.24 grams of barium acetate (Ba(C_{2}H_{3}O_{2})_{2} + H_{2}O) or 9.15 grams of barium chlorid (BaCl_{2} + 2H_{2}O) per diem. This amount daily administered would, theoretically, readily produce chronic poisoning owing to the accumulation in the system, as was shown in the case of rabbits. There is, however, some question as to whether this full theoretical amount of loco plants is eaten on the range, and the estimate has been made that one-sixth of this amount only would be actually taken. It must be remembered, as Stalker pointed out, that locoed animals develop an especial taste for these plants and after a time reject other food, so that while the number of loco plants at first taken may be small, yet later, perhaps, it is greater. A part of this barium, however, may not be taken up by the system, but may pass out undissolved. No actual experiments have yet been made with cattle by feeding small doses of the pure salt. No doubt more of the pure barium salts will be required to produce symptoms of poisoning in animals than would be necessary in the case of the form of barium found in the plant, as in the loco-weed the barium is probably better protected from precipitation than are the barium salts when dissolved in water alone. * * * * * FOOTNOTES: [250] Lane, C. B. Soiling Crop Experiments. N. J. Agric. Exper. Sta. Bul. 158, p. 18. 1902.--Woll, F. W. One Hundred American Rations for Dairy Cows. Univ. Wis. Agric. Exper. Sta. Bul. 38, p. 12. 1894.--N. J. State Agric. Exper. Sta., 20th Ann. Rept. (1899), p. 193. 1900. CONCLUSIONS.[251] (1) Conditions analogous to those met with in locoed animals occur in other portions of the world, especially Australia. (2) The main symptoms described in stock on the range can be reproduced on rabbits by feeding extracts of certain loco plants. Those especially referred to here under the term "loco plants" are _Astragalus mollissimus_ and _Aragallus lamberti_. (3) The production of chronic symptoms in rabbits is a crucial test of the pharmacological activity of these plants. (4) The inorganic constituents, especially barium, are responsible for this action, at least in the plants collected at Hugo, Colo. Perhaps in other portions of the country other poisonous principles may be found.