The amount of injected adrenin used to produce adrenin glycosuria is enormous. Osgood has studied in the Harvard Physiological Laboratory the effects on blood pressure of alternately stimulating the left splanchnic nerves (with the splanchnic vessels eliminated) and injecting adrenin, and by this method of comparison[141] has shown that the amount secreted after five seconds of stimulation varies between 0.0015 and 0.007 milligram. If 0.005 milligram is taken as a rather high average figure, and doubled (for two glands), the amount would be 0.01 milligram. To produce adrenin glycosuria, an animal weighing two kilos would be injected with two hundred times this amount. It is granted that more adrenin would be secreted if the nerves were stimulated longer than five seconds, and that with injection under the skin or into the abdominal cavity (to produce glycosuria), the amount of adrenin in the blood at one time would not be so great as if the injection were into a vein; but even with these concessions the amount of adrenin in the blood, when it has been injected to produce glycosuria, is probably very much above the amount following physiological stimulation of the glands. Other evidence that the amount of adrenin discharged when the glands are stimulated is not so great as the amount needed to produce glycosuria when acting alone is presented in experiments by Macleod.[142] He found that if the nerve fibres to the liver were destroyed, stimulation of the splanchnic, which would cause increased adrenal secretion, did not increase the blood sugar. The increased blood sugar due to splanchnic stimulation, therefore, is a nervous effect, dependent, to be sure, on the presence of adrenin in the blood, but the amount of adrenin present is not in itself capable of evoking increase. Furthermore, the increased blood sugar following splanchnic stimulation may long outlast the stimulation period. The adrenals, however, as has been demonstrated by Osgood, are soon fatigued, and fail to respond to repeated stimulation. They seem to be incapable of prolonged action. Again, as Macleod[143] has shown, a rise in the sugar content of the blood can be induced, if the adrenals are intact, merely by stimulating the nerves going to the liver. The increased blood sugar of splanchnic origin, therefore, is not due to a disturbance of the _use_ of sugar in the body, as Wilenko claims for the increase after adrenin injection, but is a result of a breaking down of the stored glycogen in the liver and is of nervous origin.