Another method which has been generally adopted for military work during
the war, consists in the addition of definite volumes of a standard
chlorine solution to several samples of the water and, after a definite
interval, testing for the presence of free chlorine by the
starch-iodide reaction. The details of the method of Gascard and
Laroche, which is used by the French sanitary service, have been given
by Comte.[12] One hundred c.cms. of the water to be examined are placed
in each of 5 vessels and 1, 2, 3, 4, and 5 drops of dilute Eau de
Javelle (1:100) are added and the contents stirred. After twenty
minutes, 1 c.cm. of potassium iodide-starch reagent (1 gram each of
starch, potassium, iodide and crystallized sodium carbonate to 100
c.cms.) is added and the samples again stirred. The lowest dilution
showing a definite blue colour is regarded as the dose required, and the
number of drops is identical with that required of the undiluted Eau de
Javelle for 10 litres of water when the same dropping instrument is
used. The actual concentration represented by these dilutions depends
necessarily upon the size of the drops and the strength of the undiluted
Eau de Javelle, but one drop per 100 c.cms. usually represents
approximately 1 p.p.m.
[Illustration: DIAGRAM V
RELATION OF DOSAGE TO OXYGEN ABSORBED]
In Horrocks's method, as used in the British army, a standard bleach
solution is added and is almost immediately followed by the zinc
iodide-starch reagent. The two methods were compared by Massy,[13] who
found that the French method gave an average result of only 0.06 m.gr.
per litre (0.06 p.p.m.) higher than the English method. Water in the
Gallipoli campaign required from 0.21 to 1.06 p.p.m. as determined by
both methods.
Diénert, Director of the Paris Service for investigating drinking water,
adds 3 p.p.m. of available chlorine and allows the mixture to stand
fifteen minutes after shaking; the residual chlorine is then titrated
with thiosulphate. The amount absorbed is increased by 0.5 p.p.m. and in
the opinion of Diénert this dosage is correct for a contact period of
three hours.
For military camps where a standpipe usually provides a reasonable
contact period, it has been found good practice to add sufficient
chlorine to give a rich blue colour with the starch-iodide reagent and
subsequently reduce the dosage gradually until the water, after standing
one hour, gives but a faint reaction to the test reagent. This method
should be checked up as soon as possible by bacteriological
examinations. An example of this method is given in Table XIV.
TABLE XIV.--CONTROL OF DOSAGE BY STARCH-IODIDE REACTION
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