The simple method above detailed will answer most ordinary purposes, provided that a few precautions be attended to. The chief are as follows. The outside of the block of tissue must be sufficiently dry for the wax-and-oil to adhere to it. The razor must be extremely sharp, and must be kept so by application to a Turkey stone during the section-cutting. The blade must be drawn across the tissue from heel to point, and kept wetted with spirit the whole time, so as to prevent any dragging of the section. The transference of the section to the slide must be effected by means of a section-lifter, which may be made by beating out a piece of stout copper wire to a thin flat blade; or a small palette-knife, or German-silver lifter, may be purchased for a few pence. The carbolic turpentine is best used by placing a little in a watch-glass, and floating the sections on to it by lifting them singly with the lifter, freeing them from superfluous spirit by draining on to blotting-paper, and allowing them to float on to the surface of the liquid in the watch-glass, so that the spirit may evaporate from above, and be replaced by the clearing agent from below The balsam solution should be thin, and the cover-glass must be allowed to settle down into place without pressure. The question of staining sections is a very large one, and is becoming of daily increasing complexity. We cannot go into it here, further than to say that most sections cut from unstained tissue will yield excellent results if stained first with Delafield’s logwood solution (to be obtained at Baker’s) to a very slight extent, and then with a solution of safranin. The sections should be washed with tap-water after the logwood stain, and should be of a pale violet colour. If over-stained, the colour may to a great extent be removed by washing with a very weak solution of hydrochloric acid, about two drops of acid to each ounce of water, and repeated washing in tap-water to remove the acid, and restore the violet. The safranin stain should be weak, and should be allowed to act for some time. From this last the sections are transferred to strong spirit, the latter being renewed until the sections cease to give up the red dye; and they may then be mounted as described. The results with most tissues are superb, every detail of the structure being splendidly brought out. Safranin alone is also an admirable stain for many purposes. Further information must be sought in the book already mentioned. Let us, in closing, warn the beginner of two things which are of general application in practical microscopy. The first is, not to be discouraged by failures. The manipulations are in many cases very delicate, and premiums must be paid to experience for insurance against failure in every one of the processes.