To get the best results, some plan of staining must be adopted. Perhaps the simplest, and certainly a very excellent one, is as follows. After the tissue has been passed from the hardening, or fixing, fluid into the successive alcohols, as described, it is placed in the following solution. Take about forty grains of carmine and eighty grains of borax, dissolve in about an ounce of water, add to the mixture an ounce of methylated spirit, and let it stand for some time with frequent shaking; about a week will be sufficient, and the process of solution may be hastened by gentle warming at intervals. The clear upper portion is then poured off, and into this the block of tissue is dropped, and allowed to remain until thoroughly penetrated. Perhaps the best plan is to substitute the carmine solution for the 50 per cent. alcohol, and thus to make the staining a stage in the hardening process. From the carmine solution the tissue is transferred to 70 per cent. alcohol, to each ounce of which two drops of hydrochloric acid have been added, and after remaining in it for a day (with a piece of the usual size) is placed in 70 per cent. alcohol, in two successive quantities. Sections from this material now only require treatment with the carbolic acid and turpentine above mentioned to be fit for mounting and covering in balsam. We now proceed to indicate how the sections may be cut. A mixture of wax and almond oil, in proportions varying with the heat of the weather, usually about equal ones, is prepared. The piece of tissue is freed from superfluous spirit by being placed on a bit of blotting-paper for a minute or two, and is then immersed in a quantity of the wax-and-oil mixture contained in a little box of paper or lead-foil. The tissue is held on the point of a needle, and lifted up and down until it is coated with the mixture, and, before solidification of the mass sets in, is lowered into the box and left to cool. The block now furnishes a handle, and this should be wrapped round with paper, the sections cut with the keenest possible razor, and as thin as possible, and placed in spirit as cut. From the spirit, which must be the strongest obtainable, they are placed in the clearing liquid, carbolic and turpentine, and then slid on to the slide, a drop of balsam placed on the section, and the cover over all. Of late years all sections of ordinary soft tissues, animal or vegetable, have been cut by one of the infiltration methods, in which the interstices of the tissue are filled up by some material which prevents the relations of the cells from being altered during the process of cutting. To enter fully into this matter would occupy too much space, and would serve no useful purpose, for the worker who requires to make use of such means will find it indispensable to obtain Bolles Lee’s _Microtomist’s Vade Mecum_, in which the whole matter is exhaustively treated.