Common objects of the microscopeWood, J. G. (John George)
Science
Common objects of the microscope
Wood, J. G. (John George)
Microscopes; Microscopy
To reduce the problem to its most simple elements, turn the mirror
altogether out of the way, and place the microscope upon a block at
such a height as shall be convenient for observation, and shall allow
the rays from the lamp, placed in a line with it on the table, to shine
directly into the tube of the microscope. Ascertain that this is so
by removing both objective and eye-piece and looking down the tube,
when the flame should be seen in the centre, edgewise. Now replace
the eye-piece, and screw on to the tube the one-inch combination or
objective. Place upon the stage an object, preferably a round diatom or
an echinus-spine, and focus it as sharply as possible. Now place the
substage condenser in its jacket, and slide it up and down until the
image of the object is bisected by the image of the flame.
The centre of the object will now be brilliantly illuminated by rays
travelling in the proper direction for yielding the best results.
The object is situated at the common focus of the microscope and the
condenser, and, whatever means of illumination be adopted, this is the
result which should always be aimed at.
Satisfactory as this critical arrangement is, however, from a
scientific point of view, it has its drawbacks from an artistic and
æsthetic one. It is not pleasant, for most purposes, to have merely the
centre of an object lighted up, and we have now to consider how the
image of the edge of the flame may be so expanded as to fill the field
without sacrificing more than a very small fraction of the accuracy of
the arrangement just attained.
Referring to Fig. 1, we see that if we place the lamp at the principal
focus of a lens, it will emit a bundle of parallel rays equal in
diameter to the diameter of the lens. This is the key of the position.
We cannot place the lamp at an infinite distance from the substage
condenser, but we can supply the latter with rays approximately
parallel, so that it shall bring them to a focus upon the object
at very nearly its own principal focus. This we do by means of the
bull’s-eye condenser. Place the latter, with its flat side toward the
edge of the flame, and at its principal focal distance (the method of
determining which has already been described) from the latter, so that
the bundle of parallel rays which issue from it may pass up to the
substage condenser. On examining the object again, it will be found
that, after slight adjustments of the position of the bull’s-eye have
been made, the object lies in the centre of an evenly and brilliantly
lighted field.
Public-domain text, read in full here on John Shaqi.
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