which is obtained by adding ammonia to the readily procurable salt
hydrogen sodium racemate. Sodium ammonium racemate was found by Pasteur
to be decomposable into the salts of dextro and lævo tartaric acids, on
crystallisation of a solution saturated at 28° C. by inoculation with a
crystal of either of those active salts. The solution on cooling being
in the state of slight supersaturation, which we now know from the work
of Ostwald and of Miers as the metastable condition, corresponding to
the interval between the solubility and supersolubility curves (see Fig.
98), if a crystal say of sodium ammonium lævo-tartrate be introduced,
this variety crystallises out first and can be separated from the
residual dextro-salt, which can then be subsequently crystallised.
Moreover, in certain direct crystallisations of sodium ammonium racemate
without such specialised inoculation, Pasteur found all the crystals
hemimorphic, some right-handed and some left-handed, and he was actually
able to isolate from each other crystals of the two varieties. On
separate recrystallisation of these two sets of crystals, he found them
to retain permanently their right or left-handed character, indicating
that the molecules themselves composing these crystals were
enantiomorphous. Their solutions correspondingly rotated the plane of
polarisation of light in opposite directions. Pasteur afterwards
obtained from the dextro-salt pure ordinary (dextro) tartaric acid, and
from the lævo-salt the lævo-acid, by converting them first into the lead
salts and then precipitating the lead as sulphide by sulphuretted
hydrogen.
In the case of lævo tartaric acid, this was its first isolation, as it
had hitherto been unknown. Gernez afterwards independently found that a
saturated solution of sodium ammonium racemate affords crystals of the
lævo-salt just as readily as of the dextro-salt; if a crystal of either
salt be introduced, crystals corresponding to that variety are produced.
Another most fruitful observation of Pasteur, the principle of which has
since been the means of isolating one of the two constituents of many
racemic compounds, was that when the spores of _Penicillium glaucum_ are
added to a solution of racemic acid containing traces of phosphates the
ordinary dextro component is destroyed by the organism, while the lævo
component is unattacked so long as any dextro remains; hence, if the
fermentation operation be stopped in time the lævo-acid may be isolated
and crystallised. Why a living organism thus eats up by preference one
variety only, possessing a particular right or left-handed screw
structure, of a compound containing the same elementary constituents
chemically united in the same manner, remains a most interesting
biological mystery.
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