Mr. Darwin was of opinion that, on the whole, the largeness of the area occupied by a species was of more importance than isolation, as a factor in the production of new species, and in this I quite agree with him. It must, too, be remembered, that isolation will often be produced in a continuous area whenever a species becomes modified in accordance with varied conditions or diverging habits. For example, a wide-ranging species may in the northern and colder part of its area become modified in one direction, and in the southern part in another direction; and though for a long time an intermediate form may continue to exist in the intervening area, this will be likely soon to die out, both because its numbers will be small, and it will be more or less pressed upon in varying seasons by the modified varieties, each better able to endure extremes of climate. So, when one portion of a terrestrial species takes to a more arboreal or to a more aquatic mode of life, the change of habit itself leads to the isolation of each portion. Again, as will be more fully explained in a future chapter, any difference of habits or of haunts usually leads to some modification of colour or marking, as a means of concealment from enemies; and there is reason to believe that this difference will be intensified by natural selection as a means of identification and recognition by members of the same variety or incipient species. It has also been observed that each differently coloured variety of wild animals, or of domesticated animals which have run wild, keep together, and refuse to pair with individuals of the other colours; and this must of itself act to keep the races separate as completely as physical isolation. _On the Advance of Organisation by Natural Selection._