Figure 4A represents a transverse section through the head of an embryo
of the approximate age of the one just described; it passes through both
forebrain, _fb_, and hindbrain, _hb_; through the extreme edge of the
optic vesicles, _ov_, and through the anterior end of the notochord,
_nt_. It is just cephalad to the anterior end of the pharynx and to the
hypophysis. The chief purpose in showing this section is to represent
the two large head-cavities, _hc_. The origin of these cavities may be
discussed at a later time. They are irregularly oval in cross section,
and extend in an antero-posterior direction for a distance about equal
to their long axis as seen in cross section. The two cavities project
towards each other in the middle line, and are almost in contact with
the notochord, in the region figured, but they do not fuse at any point.
These two head-cavities are the only ones to be seen, in this animal,
unless the small evaginations from their walls represent other cavities
fused with these. Their walls are thin but distinct, and consist of a
single layer of cells. These cells are completely filled with their
large, round nuclei, so that the wall has the appearance, under higher
magnification than is used in this figure, of a band of closely strung,
round beads.
Figure 4B represents the eighteenth section caudad to the one just
described. It passes through that region of the enteron, _ph_, which may
be called the pre-oral gut, since it lies cephalad to the now open
mouth. Owing to the plane of the section the upper angle of the first
gill cleft, _g^1_, is seen on the left, although this would not
naturally have been expected in a section through the pre-oral gut. The
evagination to form the hypophysis, _p_, is seen against the floor of
the forebrain, _fb_. The wall of this region of the enteron is
comparatively thin, and consists of not more than two layers of
compactly arranged cells with round nuclei.
Figure 4C is about forty sections caudad to the one just described. It
passes through the mouth, seen as a vertical opening between the two
mandibular arches, _md_. The hyomandibular cleft, _g^1_, the only one
which opens to the exterior in this embryo, is very wide, and may be
traced through a number of sections; in this section the opening is seen
only on the left. The pharynx, _ph_, is very wide; as it is followed
caudad its ventral opening is gradually closed by the approach of the
two mandibular folds. The dorsal wall of this region of the pharynx is
very thin, consisting of a single layer of flat cells with round nuclei;
while the ventral wall, leading through the mouth and lining the
mandibular folds, is composed of two or three layers of compactly
arranged cells.
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