Directions for Collecting and Preserving InsectsRiley, Charles V. (Charles Valentine)
Science
Directions for Collecting and Preserving Insects
Riley, Charles V. (Charles Valentine)
Insects -- Collection and preservation
_Mounting Specimens for the Microscope._--The study of the minuter forms
of insect life, including Parasites, Thysanura, Mallophaga, the newly
hatched of most insects, etc., requires the use of the microscope, and
some little knowledge of the essentials of preparing and mounting
specimens is needed. The subject of mounting the different organs of
insects and the preparation for histological study of the soft parts of
insects opens up the immense field of microscopy, the use of the
innumerable mounting media, the special treatment of the objects to be
mounted, staining, section-cutting, and many other like topics, a full
description of which is altogether out of place in the present work.
Anyone desiring to become thoroughly versed in the subject should
consult some of the larger manuals for the microscopist, of which there
are many. For the practical working entomologist, however, a knowledge
of all these methods and processes is not essential, and in my long
experience I have found that mounting in Canada balsam will answer for
almost every purpose. The softer-bodied forms will shrink more or less
in this substance, and it is frequently necessary to make studies or
drawings of them when freshly mounted; or, if additional specimens are
preserved in alcohol, they will supplement the mounted specimens and the
material may be worked up at the convenience of the student. The
materials for the balsam mounts may be obtained of any dealer in
microscopical supplies. They consist of glass slides, 3 inches by 1
inch, thin cover-glasses of different dimensions, and the prepared
balsam. The balsam is put up very conveniently for use in tin tubes. A
sufficient quantity is pressed out on the center of the glass slide,
which has previously been made thoroughly clean and dry, the insect is
removed from the alcohol, and when the excess of liquor has been removed
with bibulous paper, it is placed in the balsam, the limbs and antennae
being arranged as desired by the use of fine mounting-needles. A
cover-glass, also made thoroughly clean and dry, is then placed over the
specimen and pressed gently until the balsam entirely fills the space
between the cover and the glass slide. The slide should then be properly
labeled with a number referring to the notes on the insect, preferably
placed on the upper edge of the slide above the cover-glass, and also a
label giving the number of the slide and the number of the slide box. On
the opposite end of the slide may be placed the label giving the name of
the specimen mounted and the date. If a revolving slide table is
employed to center the mounts, the appearance of the slide may be
improved by adding a circle of asphalt or Brunswick black. With the
balsam mounts, however, this sealing is not necessary. The slide (Fig.
110) should then be placed in a slide case with the mount uppermost, and
should be kept in a horizontal position to prevent sliding of the
cover-glass and specimen until the balsam is thoroughly dried. For
Public-domain text, read in full here on John Shaqi.
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