Directions for Collecting and Preserving InsectsRiley, Charles V. (Charles Valentine)
Science
Directions for Collecting and Preserving Insects
Riley, Charles V. (Charles Valentine)
Insects -- Collection and preservation
The simplest and quickest, but perhaps the least satisfactory, method is
to remove the scales with a camel's-hair brush. This will answer for the
larger forms and where a very careful examination is not required. For
more careful examination and study the wings are first bleached by the
action of some caustic solution and then mounted in balsam for permanent
preservation. Chambers's method for Tineina, Tortricina, Pyralidina, and
the smaller moths generally, is as follows: The wing is placed on a
microscopic slide in from 3 to 4 drops of a strong solution of potash,
the amount varying according to the size of the wing. A cover of glass
is then placed in position on the wing as in ordinary mounting.
The quantity of liquid should be sufficient to fill the space beneath,
but not sufficient to float the cover glass. The mount is then placed
over an alcohol flame, removing it at the first sign of ebullition, when
the wing will be found denuded, if it be a fresh specimen. An old
specimen, or a larger wing, will require somewhat more prolonged
boiling. The fluid is drawn off by tilting the glass or with bibulous
paper, and the potash removed by washing with a few drops of water. The
cover glass is then removed and the wing mounted either on the same
slide in balsam or floated to another slide, or at once accurately
sketched with the camera lucida. Permanent mounting, however, is always
to be recommended.
The Dimmock method of bleaching the wings of Lepidoptera, given in
Psyche, Vol. I, pp. 97-99, is as follows: He uses for bleaching a
modification of the chlorine bleaching process commonly employed in
cotton bleacheries, the material for which is sold by druggists as
chlorate of lime. The wings are first soaked in pure alcohol to dissolve
out the oily matter, which will act as a repellant to the aqueous
chlorine solution. The chlorate of lime is dissolved in 10 parts of
water and filtered. The wings are transferred to a small quantity of
this solution and in an hour or two are thoroughly bleached, the veins,
however, retaining a light brown color. If the bleaching does not
commence readily in the chlorine solution the action may be hastened by
previously dipping them in dilute hydrochloric acid. When sufficiently
decolorized the wings should be washed in dilute hydrochloric acid to
remove the deposit of calcic carbonate, which forms by the union of the
calcic hydrate solution with the carbonic dioxide of the air. The wings
are then thoroughly washed in pure water and may be gummed to cards or
mounted on glass slides in Canada balsam, first washing them in alcohol
and chloroform to remove the moisture. If either of the solutions known
as _eau de labaraque_ and _eau de javelle_ are used in place of the
bleaching powder, no deposit is left on the wings and the washing with
acid is obviated. This process does not dissolve or remove the scales,
but merely renders them transparent, so that they do not interfere with
the study of the venation.
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