Directions for Collecting and Preserving InsectsRiley, Charles V. (Charles Valentine)
Science
Directions for Collecting and Preserving Insects
Riley, Charles V. (Charles Valentine)
Insects -- Collection and preservation
Prof. C. H. Fernald (_American Monthly Microscopical Journal_, I, p.
172, 1880), mounts the wings of Lepidoptera in glycerin, after having
first cleared them by the Dimmock process. After bleaching and washing,
the wings are dried by holding the slides over an alcohol flame, and a
drop of glycerin is then applied and a cover glass put on at once. By
holding the slide again over the flame until ebullition takes place the
glycerin will replace the air under the wings and no injury to the
structure of the wings will result, even if, in refractory cases, the
wing is boiled for some little time. The mount in this method must be
sealed with some microscopic cement, as asphalt or Brunswick black.
A method of mounting wings of small Lepidoptera for studying venation,
which I have found very convenient, is thus described by Mr. Howard in
_Insect Life_, Vol. I, p. 151:
"Some years ago we used the following method for studying the venation
of the wings of small Lepidoptera. We have told it since to many
friends, but believe it has not been published. It is in some respects
preferable to the so-called 'Dimmock process,' and particularly as a
time-saver. It is also in this respect preferable to denudation with a
brush. The wing is removed and mounted upon a slide in Canada balsam,
which should be preferably rather thick. The slide is then held over the
flame of an alcohol lamp until the balsam spreads well over the wing.
Just as it is about to enter the veins, however, the slide is placed
upon ice, or, if in the winter time, outside the window for a few
moments. This thickens the balsam immediately and prevents it from
entering the veins, which remain permanently filled with air and appear
black with transmitted light. With a little practice one soon becomes
expert enough to remove the slide and cool it at just the right time,
when the scales will have been rendered nearly transparent by the
balsam, while the veins remain filled with air. We have done this
satisfactorily not only with Tortricidae and Tineidae, but with Noctuids
of the size of _Aletia_ and _Leucania_. The mounts are permanent, and we
have some which have remained unchanged since 1880. Prof. Riley had for
some years before this been in the habit of mounting wings in balsam, in
which of course the scales cleared after a time."
Prof. John B. Smith recommends a modification of the Dimmock process of
bleaching the wings of Lepidoptera, publishing it in Insect Life, Vol.
I, pp. 291, 292, as follows:
Public-domain text, read in full here on John Shaqi.
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