=10.= Let us now take some fresh living threads and mount them in water. Place a small drop of dilute glycerine on the slip at one side of the cover glass, and with a bit of filter paper at the other side draw out the water. The glycerine will flow under the cover glass and come in contact with the spirogyra threads. Glycerine absorbs water promptly. Being in contact with the threads it draws water out of the cell cavity, thus causing the layer of protoplasm which lines the inside of the cell wall to collapse, and separate from the wall, drawing the chlorophyll band inward toward the center also. The wall layer of protoplasm can now be more distinctly seen and its granular character observed. We have thus employed three tests to demonstrate that this substance with which we are dealing shows the reactions which we know by experience to be given by protoplasm. We therefore conclude that this colorless and partly granular, slimy substance in the spirogyra cell is protoplasm, and that when we have performed these experiments, and noted carefully the results, we have _seen_ protoplasm. [Illustration: Fig. 4. Cell of spirogyra before treatment with glycerine.] [Illustration: Fig. 5. Cells of spirogyra after treatment with glycerine.] =11. Earlier use of the term protoplasm.=—Early students of the living matter in the cell considered it to be alike in substance, but differing in density; so the term protoplasm was applied to all of this living matter. The nucleus was looked upon as simply a denser portion of the protoplasm, and the nucleolus as a still denser portion. Now it is believed that the nucleus is a distinct substance, and a permanent organ of the cell. The remaining portion of the protoplasm is now usually spoken of as the _cytoplasm_. In spirogyra then the cytoplasm in each cell consists of a layer which lines the inside of the cell wall, a nuclear layer, which surrounds the nucleus, and radiating strands which connect the nucleus and wall layers, thus suspending the nucleus near the center of the cell. But it seems best in this elementary study to use the term protoplasm in its general sense. Protoplasm in mucor. =12.= Let us now examine in a similar way another of the simple plants with the special object in view of demonstrating the protoplasm. For this purpose we may take one of the plants belonging to the group of _fungi_. These plants possess no chlorophyll. One of several species of _mucor_, a common mould, is readily obtainable, and very suitable for this study.[3]