Test the cotyledon of the bean with iodine for the presence of
starch. If the endosperm of corn seed has not been tested do so now
with iodine. The endosperm consists largely of starch. The starch is
converted to glucose by a diastatic “ferment” formed by the seedling as
it germinates. Make a thin cross-section of a grain of wheat, including
the seed coat and a portion of the interior, treat with iodine and
mount for microscopic examination. Note the abundance of starch in the
internal portion of endosperm. Note a layer of cells on the outside of
the starch portions filled with small bodies which stain yellow. These
are aleurone grains. The cellulose in the cell walls of the endosperm
is dissolved by another enzyme called _cytase_, and some plants store
up cellulose for food. For example, in the endosperm of the _date_ the
cell walls are very much thickened and pitted. The cell walls consist
of reserve cellulose and the seedling makes use of it for food during
growth.
=216=_c_. =Albuminous and exalbuminous seeds.=—In seeds
where the food is stored outside of the embryo they are called
_albuminous_; examples, corn, wheat and other cereals, Indian turnip,
etc. In those seeds where the food is stored up in the embryo they are
called _exalbuminous_; examples, bean, pea, pumpkin, squash, etc.
=217. Digestion= has a well-defined meaning in animal physiology
and relates to the conversion of solid food, usually within the
stomach, into a soluble form by the action of certain gastric juices,
so that the liquid food may be absorbed into the circulatory system.
The term is not often applied in plant physiology, since the method
of obtaining food is in general fundamentally different in plants and
animals. It is usually applied to the process of the conversion of
starch into some form of sugar in solution, as glucose, etc. This we
have found takes place in the leaf, especially at night, through the
action of a diastatic ferment developed more abundantly in darkness. As
a result, the starch formed during the day in the leaves is digested
at night and converted into sugar, in which form it is transferred to
the growing parts to be employed in the making of new tissues, or it is
stored for future use; in other cases it unites with certain inorganic
substances, absorbed by the roots and raised to the leaf, to form
proteids and other organic substances. In tubers, seeds, parts of stems
or leaves where starch is stored, it must first be “digested” by the
action of some enzyme before it can be used as food by the sprouting
tubers or germinating seeds.
For example, starch is converted to a glucose by the action of a
diastase. Cellulose is converted to a glucose by cytase. Albuminoids
are converted into available food by a tryptic ferment. Fatty oils are
converted into glucose and other products by lipase.
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