Epidemic Respiratory Disease: The pneumonias and other infections of the repiratory tract accompanying influenza and measlesOpie, Eugene L. (Eugene Lindsay)
History
Epidemic Respiratory Disease: The pneumonias and other infections of the repiratory tract accompanying influenza and measles
=Bacteriologic Methods Used in the Study.=—The methods used for the
identification of hemolytic streptococci here were essentially the same
as those used at Camp Funston and described above, the one exception
being the use of surface cultures on blood agar instead of the combined
surface and deep culture. Blood agar plates containing 5 per cent
defibrinated horse blood were poured and used while fresh. The throat
swabs were carried to the laboratory in sterile test tubes. The plates
were inoculated by touching the swab lightly to the surface of the agar
plate at two places, one near either extremity of a given diameter of
the plate. On touching the swab to the agar, the swab stick was rolled
between the fingers so as to turn it through one revolution and thereby
bring all points of the circumference of the cotton swab in contact with
the agar surface.
The material thus inoculated on the plates was spread by means of a
platinum wire slightly turned over at the end in “hockey stick” fashion.
The wire was passed back and forth several times over the point of
inoculation and then multiple streaks and cross streaks were made over
the agar surface. The initial contact of the wire with the point of
inoculation was not repeated. The cross streaking serves to spread and
distribute this material evenly over the surface. Well seeded plates by
this multiple streak method are the rule and the uniform distribution of
well separated colonies over the surface makes it very easy to pick pure
cultures, and renders plate reading easy.
Very early in the course of our study of throat cultures at Camp Pike,
the great frequency of abundant growths of B. influenzæ was observed.
Consequently, the throat cultures of all measles patients examined from
September 15 to October 20 were studied for the identification of B.
influenzæ. In all cases identification was based on the cultural,
staining and morphologic characteristics. Tests for growth on hemoglobin
free media were not made as a routine.
=Relation of Measles and Pneumonia Following Measles to the Influenza
Epidemic.=—The influenza epidemic at Camp Pike was recognized on
September 23 because of an alarming increase of hospital admissions. It
ran its brief course, and ten days later, October 3, the decline began.
The first four days of October rank highest in admissions of patients
with pneumonia following influenza. The onset of 20 scattered cases of
measles occurred before September 25, and later the number slowly
increased reaching its height about the middle of October; after this
time a gradual decline began, and continued during about three weeks
before the preepidemic level was reached. During this period of six
weeks following September 25, 709 cases of measles occurred.
TABLE LVIII
ONSET OF MEASLES AND OF PNEUMONIA FOLLOWING MEASLES BY WEEKS FROM
SEPTEMBER 11 TO DECEMBER 11, 1918
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