sufficient for the purposes of respiration, he plunged some partially in water, so that only two or more spiracula remained in the open air: in these cases the caterpillar did not become torpid as it did when they were all immerged in water. One caterpillar, upon which Bonnet made his experiments, lived eight days suspended in water, with only two of its anterior spiracula in the air; during this time he observed, that when the insect moved itself, little streams of bubbles issued from the anterior spiracula on the left side; from this, and many other experiments, it appeared to him, that amongst all the eighteen spiracula, the two anterior and the two posterior are of the greatest use in respiration.[79] Sometimes when the apertures of these have been stopped with oil, the caterpillar has fallen into convulsions. If the posterior part had been oiled, that part became paralytic. Notwithstanding these experiments, and many more which have been made, the subject is far from being decided, and many still doubt whether there is any respiration in insects similar to ours, at least at certain periods of their life. This opinion seems to be further confirmed by the experiments of M. Lyonet. He confined several large musk beetles under a glass for more than half an hour, exposed to the fumes of burning sulphur; and, though during their continuance there the vapour was so thick that he could not see them, yet on their being liberated, they did not seem at all effected thereby.[80] [79] Philos. Trans. vol. xlv. p. 300. [80] Lesser Theologie des Insectes, tom. 1, p. 124. Ibid. p. 126. Supposing respiration to be absolutely necessary to the existence of the pupæ of different insects, when we reflect on the great solidity of their cases or cones, it is not easy to conceive how they can live several months under the earth, in spaces so confined, and almost impervious to the air: and indeed if they did respire, the same situation seems to preclude a continuance of the operation, as the air would soon be corrupted, and unfit for the offices of life. As the tracheæ are divided and subdivided to a prodigious degree of minuteness, it has been conjectured by some writers, that they may act as so many sieves, which, by separations properly contrived, filtrate the air, and so furnish it to the body of different degrees of purity and subtilty, agreeable to the purposes and nature of the various parts. The experiments that have been made with the air-pump are by no means conclusive; the injury which the insect sustains when the atmospheric pressure is taken from the body, does not prove that it inspired and expired the air that we have removed; it only shews that an incumbent pressure is necessary to their comfortable existence, as it prevents the fluids from disengaging themselves in an aerial form, and as it counterbalances and re-acts on the principle of life, and, by keeping the action thereof in proper tone and order, confines and regulates its energies.