Though it is difficult to ascertain whether some insects respire, at least at certain periods of their existence, yet there are others to whom the inspiration and expiration of air seems absolutely necessary: there are many aquatic insects which are obliged to keep their tails suspended on the surface of the water for this purpose. To prove this, keep the tail under water, and you will perceive the insect to be agitated and uneasy, and to seek for some opening to expose this part to the air; if it find none, it soon goes to the bottom and dies. Some aquatic beetles resist the trial for a considerable time, while their larvæ can only support it for a few minutes. There is a circumstance which renders all experiments on this subject with insects doubtful and difficult, namely, the vast tenaciousness of the life principle in the lower orders of animated nature, and its dissemination through their whole frame. Musschenbroeck inclosed the pupa of a moth in a glass tube, very little larger than the moth itself, and of the following figure. [Illustration] The end A of the tube was drawn out in a capillary form, the other end was covered with a piece of wet bladder to exclude the air; the capillary end B was then plunged in water, which rose to D. He placed the capillary part of the tube before a microscope, on a small micrometer, in order to observe any motion or change in the situation of the water; as it is evident the expiration or inspiration of air by the insect would make it rise or fall alternately. In the first experiment he observed a small degree of motion at distant intervals, not above two or three times in an hour; in a second experiment on another subject, he could observe no motion at all. He then placed some pupæ under the receiver of an air-pump, in water which he had previously purged of its air; on exhausting the air from the receiver, he observed one bubble to arise in a part near the tail, and a few near the wings. The pupæ did not swell under the operation; on the contrary, on letting in the air, it was found to be diminished in a small degree, but in less than a quarter of an hour it recovered its former figure. M. Martinet published at Leyden, in 1753, a dissertation, in which, it is said, he has clearly proved by a number of experiments that the pupæ of caterpillars and some other insects do not respire. OF RESPIRATION IN THE LARVA OF THE MUSCA PENDULA.