After thirty-six hours infusion they were all sweet, except the infusion of Columbo in distilled water, which began to emit a disagreeable, though not putrid foetor. The beef in it, and in the tincture of the same root in lime water, was swelled, and whiter than before infusion. That in the tincture of bark prepared with quick-lime, had its texture greatly destroyed, was of a chocolate colour, but sweet. That in aqua calcis, the same in colour, shrivelled, firm, and sweet. The pieces of beef in the tinctures of snake root and of contrayerva with quick-lime, had more the appearance of calf's lights than of beef, were quite spongy, but had acquired no putrid smell. Those in the lime and distilled water, firm, and shewing no signs of putrescency. On the fifth day the infusion of Columbo in lime water was very offensive, though the beef when taken out of it was not putrid. That of the same root with distilled water had made no further progress. The tincture of snake root in distilled water was grown turbid, and had lost colour, which it seemed to have imparted to the beef. This and all the others continued sweet. On the tenth day the beef in the distilled water and Columbo, as on the fifth. That in the lime water and Columbo, putrid. The contrayerva infusion in distilled water had acquired a disagreeable foetor, but the beef was not yet putrid. That with lime water and that with quick-lime still sweet. The infusion of bark with distilled water smelled rather musty; the beef in it sweet. The two infusions of the same with lime and lime water shewed no further change. The tincture of snake root in distilled water had a scum on the surface; beef not putrid. The other two tinctures of the same root unchanged. On the eleventh day, the beef in the infusions of Columbo and of contrayerva in distilled water beginning to putrefy, and On the fourteenth day, both entirely putrid. The infusion of bark in distilled water mouldy, but the beef sweet. The beef in the snake root and distilled water, putrid on the sixteenth day; and the infusion of contrayerva with lime water beginning to be offensive, but the beef in it not yet putrid; but On the nineteenth it was quite putrefied. The snake root infusion in lime water, mouldy on its surface; no change in the beef; but this likewise became putrid in a few days more. The remaining tinctures, viz. those of the bark, snake root, and contrayerva with quick-lime, and that of the bark with lime water, remained above five weeks without any further change. Some time after, the beef in the snake root became septic. The other three were unaltered at the end of six weeks from their first immersion; and though the infusion of bark in distilled water was very mouldy, the beef in it was free from any putrid foetor. But it should be observed that all the tinctures in the preparation of which quick-lime had been added to the lime water, had a peculiar odour during the whole time, from which the others were exempt.