"Three pigs were destined to clear up my doubts. The first, six weeks old, was fed for a month on ordinary food, with an ounce daily of madder-juice--_garence grappe_--put in it. At the end of the month, we stopped the juice, and fed the pig in the ordinary way for six weeks, and then killed it. The marrow of the bones was surrounded by a fairly thick layer of white bone: this was the formation of bone during the first six weeks of life, without madder. This ring of white bone was surrounded by another zone of red bone: this was the formation of bone during the administration of the madder. Finally, this red zone was covered with a fairly thick layer of white bone: this was the layer formed after the madder had been left off.... We shall have no further difficulty in understanding whence transudes the osseous juice that was thought necessary for the formation of callus and the filling-up of the wounds of the bones, now we see that it is the periosteum that fills up the wounds, or is made thick round the fractures, and afterward becomes of the consistence of cartilage, and at last acquires the hardness of bones." [4] "An Account of the Bones of Animals being changed to a Red Colour by Aliment only," by John Belchier, F.R.S., _Phil. Trans. Roy. Soc._, 1735-36. There is a letter from Sir Hans Sloane, then President of the Royal Society, to M. Geoffroy, member of the French Academy:--"M. Belchier, chirurgien, membre de cette Société, dînant un jour chez un Teinturier qui travaille en Toiles peintes, remarqua que dans un Porc frais qu'on avoit servi sur table, et dont la chair étoit de bon goût, les os étoient rouges. Il demanda la cause d'un effet si singulier, et on lui dit que ces sortes de Teinturiers se servoient de la racine de Rubia Tinctorum, ou garence, pour fixer les couleurs déjà imprimées sur les Toiles de coton, qu'on appelle en Angleterre callicoes." This passage of dye into the bones of animals had been noted so far back as 1573, by Antoine Mizald, a doctor in Paris--_Erythrodanum, vulgo rubia tinctorum, ossa pecudum rubenti et sandycino colore imbuit._