Note first what appear to be exceptions. Certain minute animal forms present us either with no appreciable differentiations or with differentiations so obscure as to be made out with great difficulty. In the Rhizopods, the substance of the jelly-like body remains throughout life unorganized, even to the extent of having no limiting membrane; as is proved by the fact that the thread-like processes protruded by the mass, coalesce on touching each other. Whether or not the nearly allied _Amœba_, of which the less numerous and more bulky processes do not coalesce, has, as lately alleged, something like a cell-wall and a nucleus, it is clear that the distinction of parts is very slight; since particles of food pass bodily into the inside through any part of the periphery, and since when the creature is crushed to pieces, each piece behaves as the whole did. Now these cases, in which there is either no contrast of structure between exterior and interior or very little, though seemingly opposed to the above inference, are really very significant evidences of its truth. For what is the peculiarity of this division of the _Protozoa_? Its members undergo perpetual and irregular changes of form—they show no persistent relation of parts. What lately formed a portion of the interior is now protruded, and, as a temporary limb, is attached to some object it happens to touch. What is now a part of the surface will presently be drawn, along with the atom of nutriment sticking to it, into the centre of the mass. Either the relations of inner and outer have no permanent existence, or they are very slightly marked. But by the hypothesis, it is only because of their unlike positions with respect to modifying forces, that the originally like units of a living mass become unlike. We must therefore expect no established differentiation of parts in creatures which exhibit no established differences of position in their parts; and we must expect extremely little differentiation of parts where the differences of position are but little determined—which is just what we find. This negative evidence is borne out by positive evidence. When we turn from these proteiform specks of living jelly to organisms having an unchanging distribution of substance, we find differences of tissue corresponding to differences of relative position. In all the higher _Protozoa_, as also in the _Protophyta_, we meet with a fundamental differentiation into cell-membrane and cell-contents; answering to that fundamental contrast of conditions implied by the terms outside and inside. On passing from what are roughly classed as unicellular organisms, to the lowest of those which consist of aggregated cells, we equally observe the connection between structural differences and differences of circumstance. Negatively, we see that in the sponge, permeated throughout by currents of sea-water, the indefiniteness of organization corresponds with the absence of definite unlikeness of