conditions: the peripheral and central portions are as little contrasted in structure as in exposure to surrounding agencies. While positively, we see that in a form like the _Thalassicolla_, which, though equally humble, maintains its outer and inner parts in permanently unlike circumstances, there is displayed a rude structure obviously subordinated to the primary relations of centre and surface: in all its many and important varieties, the parts exhibit a more or less concentric arrangement. After this primary modification, by which the outer tissues are differentiated from the inner, the next in order of constancy and importance is that by which some part of the outer tissues is differentiated from the rest; and this corresponds with the almost universal fact that some part of the outer tissues is more exposed to certain environing influences than the rest. Here, as before, the apparent exceptions are extremely significant. Some of the lowest vegetal organisms, as the _Hematococci_ and _Protococci_, evenly imbedded in a mass of mucus, or dispersed through the Arctic snow, display no differentiations of surface; the several parts of their surfaces being subjected to no definite contrasts of conditions. Ciliated spheres such as the _Volvox_ have no parts of their periphery unlike other parts; and it is not to be expected that they should have; since, as they revolve in all directions, they do not, in traversing the water, permanently expose any part to special conditions. But when we come to organisms that are either fixed, or while moving preserve definite attitudes, we no longer find uniformity of surface. The most general fact which can be asserted with respect to the structures of plants and animals, is, that however much alike in shape and texture the various parts of the exterior may at first be, they acquire unlikenesses corresponding to the unlikenesses of their relations to surrounding agencies. The ciliated germ of a Zoophyte, which, during its locomotive stage, is distinguishable only into outer and inner tissues, no sooner becomes fixed, than its upper end begins to assume a different structure from its lower. The disc-shaped _gemmæ_ of the _Marchantia_, originally alike on both surfaces, and falling at random with either side uppermost, immediately begin to develop rootlets on the under side, and _stomata_ on the upper side: a fact proving beyond question, that this primary differentiation is determined by this fundamental contrast of conditions.