My own interference with this great question, while sanctioned by eminent names, has been also an object of varied and ingenious attack. On this point I will only say that when angry feeling escapes from behind the intellect, where it may be useful as an urging force, and places itself athwart the intellect, it is liable to produce all manner of delusions. Thus my censors, for the most part, have levelled their remarks against positions which were never assumed, and against claims which were never made. The simple history of the matter is this: During the autumn of 1868 I was much occupied with the observations referred to at the beginning of this discourse, and in part described in the preceding article. For fifteen years it had been my habit to make use of floating dust to reveal the paths of luminous beams through the air; but until 1868 I did not intentionally reverse the process, and employ a luminous beam to reveal and examine the dust. In a paper presented to the Royal Society in December, 1869, the observations which induced me to give more special attention to the question of spontaneous generation, and the germ theory of epidemic disease, are thus described: The Floating Matter of the Air. Prior to the discovery of the foregoing action (the chemical action of light upon vapours, Fragment IV.), and also during the experiments just referred to, the nature of my work compelled me to aim at obtaining experimental tubes absolutely clean upon the surface, and absolutely free within from suspended matter. Neither condition is, however, easily attained. For however well the tubes might be washed and polished, and however bright and pure they might appear in ordinary daylight, the electric beam infallibly revealed signs and tokens of dirt. The air was always present, and it was sure to deposit some impurity. All chemical processes, not conducted in a vacuum, are open to this disturbance. When the experimental tube was exhausted, it exhibited no trace of floating matter, but on admitting the air through the U-tubes (containing caustic potash and sulphuric acid), a _dust-cone_ more or less distinct was always revealed by the powerfully condensed electric beam.