The floating motes resembled minute particles of liquid which had been carried mechanically from the U-tubes into the experimental tube. Precautions were therefore taken to prevent any such transfer. They produced little or no mitigation. I did not imagine, at the time, that the dust of the external air could find such free passage through the caustic potash and sulphuric acid. This, however, was the case; the motes really came from without. They also passed with freedom through a variety of aethers and alcohols. In fact, it requires long-continued action on the part of an acid first to wet the motes and afterwards to destroy them. By carefully passing the air through the flame of a spirit lamp, or through a platinum tube heated to bright redness, the floating matter was sensibly destroyed. It was therefore combustible, in other words, organic, matter. I tried to intercept it by a large respirator of cotton-wool. Close pressure was necessary to render the wool effective. A plug of the wool, rammed pretty tightly into the tube through which the air passed, was finally found competent to hold back the motes. They appeared from time to time afterwards, and gave me much trouble; but they were invariably traced in the end to some defect in the purifying apparatus--to some crack or flaw in the sealing-wax employed to render the tubes air-tight. Thus through proper care, but not without a great deal of searching out of disturbances, the experimental tube, even when filled with air or vapour, contains nothing competent to scatter the light. The space within it has the aspect of an absolute vacuum. An experimental tube in this condition I call _optically empty_. The simple apparatus employed in these experiments will be at once understood by reference to a figure printed in the last article (Fig. 3.) s s' is the glass experimental tube, which has varied in length from 1 to 5 feet, and which may be from 2 to 3 inches in diameter. From the end s, the pipe pp' passes to an air-pump. Connected with the other end s' we have the flask F, containing the liquid whose vapour is to be examined; then follows a U-tube, T, filled with fragments of clean glass, wetted with sulphuric acid; then a second U-tube, T, containing fragments of marble, wetted with caustic potash; and finally a narrow straight tube t t', containing a tolerably tightly fitting plug of cotton-wool. To save the air-pump gauge from the attack of such vapours as act on mercury, as also to facilitate observation, a separate barometer tube was employed. Through the cork which stops the flask F two glass tubes, a and b, pass air-tight. The tube a ends immediately under the cork; the tube b, on the contrary, descends to the bottom of the flask and dips into the liquid. The end of the tube b is drawn out so as to render very small the orifice through which the air escapes into the liquid.