'When I saw your first notice respecting the poisonous action of quinine upon infusoria, I determined at once to make an experiment with that substance, thinking that these vibrionic bodies, even if they did not cause the whole illness, still could render it much more unpleasant through their movements and the decompositions caused by them. For that reason I made a neutral solution of sulphate of quinine, which did not contain much of the salt (1·800), but still was effective enough, and caused moderate irritation on the mucus membrane of the nose. I then lay flat on my back, keeping my head very low, and poured with a pipette about four cubic centimetres into both nostrils. Then I turned my head about in order to let the liquid flow in all directions. 'The desired effect was obtained immediately, and remained for some hours; I could expose myself to the sun without fits of sneezing and the other disagreeable symptoms coming on. It was sufficient to repeat the treatment three times a day, even under the most unfavourable circumstances, in order to keep myself quite free. [Footnote: There is no foundation for the objection that syringing the nose could not cure the asthma which accompanies hay fever; for this asthma is only the reflex effect arising from the irritation of the nose.--B.] There were then no such vibrios in the secretion. If I only go out in the evening, it suffices to inject the quinine once a day, just before going. After continuing this treatment for some days the symptoms disappear completely, but if I leave off they return till towards the end of June. 'My first experiments with quinine date from the summer of 1867; this year (1868) I began at once as soon as the first traces of the illness appeared, and I have thus been able to stop its development completely. 'I have hesitated as yet in publishing the matter, because I have found no other patient [Footnote: Helmholtz, now Professor of Physics at the University of Berlin, is, although M.D., no medical practitioner.--B.] on whom I could try the experiment. There is, it seems to me, no doubt, considering the extraordinary regularity in the recurrence and course of the illness, that quinine had here a most quick and decided effect. And this again makes my hypothesis very probable, that the vibrios, although of no specific form but a very frequent one, are at least the cause of the rapid increase of the symptoms in warm air, as heat excites them to lively action.