Histology of medicinal plantsMansfield, William James
Science
Histology of medicinal plants
Mansfield, William James
Botany, Medical; Plant anatomy
The =compound microscope= has undergone wonderful changes since 1667,
the days of Robert Hooke. When we consider the crude construction
and the limitations of Robert Hooke’s microscope, we marvel at the
structural perfection and the unlimited possibilities of the modern
instrument. The advancement made in most sciences has followed the
gradual perfection of this instrument.
The illustration of Robert Hooke’s microscope (Fig. 7) will convey
to the mind more eloquently than words the crudeness of the early
microscopes, especially when it is compared with the present-day
microscopes.
STRUCTURE OF THE COMPOUND MICROSCOPE
The parts of the compound microscope (Fig. 8) may be grouped
into--first, the mechanical, and, secondly, into the optical parts.
THE MECHANICAL PARTS
1. The =foot= is the basal part, the part which supports all the
other mechanical and optical parts. The foot should be heavy enough
to balance the other parts when they are inclined. Most modern
instruments have a three-parted or tripod-shaped base.
2. The =pillar= is the vertical part of the microscope attached to
the base. The pillar is joined to the limb by a hinged joint. The
hinges make it possible to incline the microscope at any angle, thus
lowering its height. In this way, short, medium, and tall persons
can use the microscope with facility. The part of the pillar above
the hinge is called the _limb_. The limb may be either straight
or curved. The curved form is preferable, since it offers a more
suitable surface to grasp in transferring from box or shelf to the
desk, and _vice versa_.
[Illustration: FIG. 7.--Compound Microscope of Robert Hooke]
3. The =stage= is either stationary or movable, round or square, and
is attached to the limb just above the hinge. The upper surface is
made of a composition which is not easily attacked by moisture and
reagents. The centre of the stage is perforated by a circular opening.
4. The =sub-stage= is attached below the stage and is for the purpose
of holding the iris diaphragm and Abbé condenser. The raising and
lowering of the sub-stage are accomplished by a rack and pinion.
5. The =iris diaphragm=, which is held in the sub-stage below the
Abbé condenser, consists of a series of metal plates, so arranged
that the light entering the microscope may be cut off completely or
its amount regulated by moving a control pin.
6. The =fine adjustment= is located either at the side or at the top
of the limb. It consists of a fine rack and pinion, and is used in
focusing an object when the low-power objective is in position, or in
finding and focusing the object when the high-power objective is in
position.
7. The =coarse adjustment= is a rack and pinion used in raising and
lowering the body-tube and in finding the approximate focus when
either the high- or low-power objective is in position.
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