Besides the _I. opaca_, _I. dahoon_, _I. cassine_, according to Curtis there are in this State five additional species of this genus: _I. decidua_ Walt. _I. ambigua_ Chapman; _I. verticillata_ Gray, _I. glabra_ Gray, _I. coriacea_ Chapm, but the examination was not extended to them. In searching for the alkaloids the directions of Dragendorff were first followed. The leaves (or crushed berries) were first digested at 40°-50° with dilute sulphuric acid. This extract was evaporated to a sirupy consistence, the residue mixed with three or four times its bulk of alcohol, filtered after 24 hours’ standing, and washed with alcohol. The alcohol was then distilled off from the filtrate, the watery residue was diluted with water and filtered. Petroleum-ether, benzol, and chloroform were successively used to extract the alkaloidal principles, if any were present in the acid liquid. Then, after rendering alkaline with ammonia, the liquid was again extracted with the solvents mentioned. As, even with water but slightly acidified with sulphuric acid, some risk of the destruction or change of the alkaloids was run during the long evaporation, a second method was made use of, as follows: The leaves were digested for 10 hours with 70 per cent alcohol, the alcohol distilled off, and the residue treated with lead acetate and soda. The excess of lead was removed by means of sulphuretted hydrogen and the filtrate from this evaporated to a thin sirup. This was then treated with strong alcohol, filtered, and the excess of alcohol distilled off. Bismuth, potassium-iodide, and sulphuric acid were next used to precipitate any alkaloid present. The presence of albuminoid matter rendered it necessary to decompose this by means of soda, neutralized with dilute sulphuric acid, and reprecipitate with mercuric chloride. The solutions to which mercuric chloride had been added were allowed to stand several days. The results may be tabulated as follows: I. opaca, leaves No alkaloid. I. opaca, berries No alkaloid. I. dahoon, leaves No alkaloid. I. dahoon, berries No alkaloid. I. cassine, leaves Caffeine. I. cassine, berries No alkaloid. I regard these analyses as conclusive, at least, of the absence of the known, well characterized alkaloids. It is, of course, possible that other methods might reveal the presence of some of the more elusive ones.