Ketchup: Methods of Manufacture; Microscopic ExaminationBitting, K. G. (Katherine Golden)
Science
Ketchup: Methods of Manufacture; Microscopic Examination
Bitting, K. G. (Katherine Golden)
Ketchup
In all biological work involving the counting of organisms, either by
the plate or direct method, in the case of yeast, the operator works as
rapidly as possible to prevent the organisms from settling, so as to
have them evenly distributed in order that he may obtain an average
sample. A pipette is used for removal of a drop of the liquid and the
drop placed in the chamber as quickly as possible to prevent settling.
No directions are given as to how the drop of the diluted pulp or
ketchup is to be removed to the chamber, so that a stirring rod or other
apparatus is frequently used, as the solid particles interfere with the
use of a fine pipette. If the rod be inserted to the bottom, or nearly
to the bottom of the mixture and withdrawn slowly and another withdrawn
somewhat rapidly, a difference of fifty per cent or even more may result
in the count. It is not possible for different operators to use
pipettes, glass rods, pen knives, toothpicks, and matches for drawing
the samples, and get comparable results. It has been found that in (all
of these have been seen in use) the counting of the organisms in pulp
and ketchup, some persons use distilled water, others tap water, some
clean their measuring flasks and pipettes, while others rinse them, so
that naturally reports are made of such varying numbers that
manufacturers do not look upon the method with confidence. It is only by
using uniform methods and the same care necessary for other biological
work that even an approximation can be made.
STRUCTURE OF THE TOMATO.
To obtain the number of yeasts and spores in the sample, a count is made
in one-half of the ruled squares. Two hundred squares represent a volume
equivalent to 1-20 c mm, which, multiplied by the dilution, would give
the number in 1-60 c mm. It is stated that it is believed that it is
possible for manufacturers to keep the count below 25 per 1-60 c mm.
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