Leaving then the question whether the Queen exercises any volition in this matter, for the present undecided, I shall state some facts which occurred in the summer of 1852, in my own Apiary, and shall then endeavor to relieve, as far as possible, this intricate subject from some of the difficulties which embarrass it. In the Autumn of 1852, my assistant found, in one of my hives, a young Queen, the whole of whose progeny was drones. The colony had been formed by removing part of the combs containing bees, brood and eggs from another hive. It had only a few combs, and but a small number of bees. They raised a new Queen in the manner which will hereafter be particularly described. This Queen had laid a number of eggs in one of the combs, and the young bees from some of them were already emerging from the cells. I perceived, at the first glance, that they were drones. As there were none but worker cells in the hive, they were reared in them, and not having space for full development, they were dwarfed in size, although the bees, in order to give them more room, had pieced out the cells so as to make them larger than usual! Size excepted, they appeared as perfect as any other drones. I was not only struck with the singularity of finding drones reared in worker cells, but with the equally singular fact that a young Queen, who at first lays only the eggs of workers, should be laying drone eggs at all; and at once conjectured that this was a case of a drone-laying, unimpregnated Queen, as sufficient time had not elapsed for her impregnation to be unnaturally retarded. I saw the great importance of taking all necessary precautions to determine this point. The Queen was removed from the hive, and carefully examined. Her wings, although they appeared to be perfect, were so paralized that she could not fly. It seemed probable, therefore, that she had never been able to leave the hive for impregnation. To settle the question beyond the possibility of doubt, I submitted this Queen to Dr. Joseph Leidy for microscopic examination. The following is an extract from his report: "The ovaries were filled with eggs; the poison sac was full of fluid, and I took the whole of it into my mouth; the poison produced a strong metallic taste, lasting for a considerable time, and at first, it was pungent to the tip of the tongue. The spermatheca was distended with a perfectly colorless, transparent, viscid liquid, _without a trace of spermatozoa_." This examination seems perfectly to sustain the theory of Dzierzon, and to demonstrate that Queens do not need to be impregnated, in order to lay the eggs of males.