Now let us look at these results carefully. The experiment with surface soil shows that the pieces of stem and leaf have furnished a good deal of food to the mustard and have caused a gain of 24.3 grams in the crop. If we knew what the pieces were made of we {51} could push the experiment still further and find out more about plant food, but this involves chemical problems and must be left alone for the present. We can, however, say that plant remains are an important source of plant food, and since we suppose the black material of the soil to be made of plant remains (see p. 36), it will be quite fair to say also that this black material, the humus, is a source of plant food. We have therefore answered the question we set, and we can explain some at any rate of the differences between the surface soil and the subsoil. The surface soil contains a great deal of the black material, which forms plant food, while the subsoil does not. Thus plants grow well on the surface soil and starve on the subsoil. We can also explain why gardeners and farmers speak of black soils as rich soils; they contain more than other soils of this black material that makes plant food. Still further, we can explain why the farmer often sows plants like mustard, tares or clover, and then ploughs them into the ground. They are not wasted, but they make food for the next crop that goes in. Now let us turn to the results of the subsoil experiments. The leaves and stems have increased the crop, but only by 5.4 grams: they have not been nearly so effective as in the surface soil. It is evident that the mustard did not feed directly on the leaves and stems put in; if it had there should have been an equal gain in both cases. The leaves and stems clearly have to undergo some change before they are made into plant food and the soil has something to do with this change. After the crops are cut the soils should be tipped out and examined. More of the original pieces of leaf and stem are found in the subsoil than in the surface {52} soil. That is to say, there has been more change in Pot 6 containing surface soil than in Pot 7 containing subsoil. The "something," whatever it may be, that changes plant remains like leaves, stems, pieces of grass, roots, etc. into plant food therefore acts better in the surface soil than in the subsoil. Here then we have another difference between surface and subsoils. SUMMARY. The experimental results obtained in this chapter may now be summed up as follows:-- (1) Plant food is present in the top soil only and not to any extent in the subsoil. (2) There is not much present, so little indeed that we could not detect it by weighing. (3) It is, however, always being made in the top soil, if water is present. Only little is made from the subsoil. (4) The remains of leaves, stems, roots, etc. furnish an important source of plant food.