In a few hours after his arrival he had already proved the presence of corpuscles in certain worms, and was able to show them to the President and several members of the Agricultural Committee, who had never seen them. The following day he installed himself in a little house three kilometers from Alais. Two small cultures were there going on; they were nearly the last, the cocoons had all been spun. One of these cultures, proceeding from eggs imported that very year from Japan, had succeeded very well. The other, proceeding also from Japanese eggs which had been reproduced in the country, had arrived at their fourth moulting and had a very bad appearance. But, strange to say, on examining with the microscope a number of chrysalides and moths of the group which had so delighted its proprietor, Pasteur found corpuscles almost always present, whereas the examination of the worms of the bad group only exhibited them occasionally. This double result struck Pasteur as very strange. He at once sent messengers into all the neighbourhood of Alais to seek for the remains of backward cultivations. He attached extreme importance to ascertaining whether the presence of corpuscles in the chrysalides or moths of the good groups, and the absence of the same corpuscles in the worms of the bad groups, was an accidental or a general fact. He soon recognised that these results did very frequently occur. But what would happen when the worms of the bad group spun their cocoons? Pasteur found that in the chrysalides, especially in the old ones, the corpuscles were numerous. As regards the moths proceeding from these cocoons, not one was free from them, and they existed in profusion. Following up the idea that a connection between the disease and the corpuscles might possibly exist, as other observers had previously imagined, Pasteur declared, in a Note presented to the Agricultural Committee of Alais on June 26, 1865, twenty days after his arrival, that it was a mistake to seek for the corpuscle in the eggs or in the worms. Both the one and the other could carry in them the germ of the disease, without exhibiting distinct corpuscles, visible under the microscope. The evil developed itself especially in the chrysalides and in the moths, and it was in them that search should be made. Finally, Pasteur came to the conclusion that the only infallible method of procuring healthy eggs must be by having recourse to moths free from corpuscles.