If the queen be observed after a successful "wedding tour," she will be seen to bear the marks of success in the pendant drone appendages, consisting of the penis, the yellow cul-de-sacks, and the hanging thread-like ducts. It is not at all likely that a queen, after she has met a drone, ever leaves the hive again except that she leaves with a swarm. Some of the observing apiarists think that an old queen may be again impregnated. The fact that queens, with clipped wings, are as long fertile as others, makes me think that cases which have led to such conclusions are capable of other explanation. If the queen lays eggs before meeting the drones, or if for any reason she fails to mate, her eggs will only produce male bees. This strange anomaly--development of the eggs without impregnation--was discovered and proved by Dzierzon, in 1845. Dr. Dzierzon, who, as a student of practical and scientific apiculture, must rank with the great Huber, is a Roman Catholic priest of Carlesmarkt, Germany. This doctrine--called parthenogenesis, which means produced from a virgin--is still doubted by some quite able bee-keepers, though the proofs are irrefragable: 1st. Unmated queens will lay eggs that will develop, but drones always result. 2d. Old queens often become drone-layers, but examination shows that the spermatheca is void of seminal fluid. Such an examination was first made by Prof. Siebold, the great German anatomist, in 1813, and later by Leuckart and Leidy. I have myself made several such examinations. The spermatheca can easily be seen by the unaided vision, and by crushing it on a glass slide, by compressing with a thin glass cover, the difference between the contained fluid in the virgin and impregnated queen is very patent, even with a low power. In the latter it is more viscid and yellow, and the vesicle more distended. By use of a high power, the active spermatozoa or germ-cells become visible. 3d. Eggs in drone-cells are found by the microscopist to be void of the sperm-cells, which are always found in all other fresh-laid eggs. This most convincing, and interesting observation, was first made by Von Siebold, at the suggestion of Berlepsch. It is quite difficult to show this. Leuckart tried before Von Siebold, at Berlepsch's apiary, but failed. I have also tried to discover these germ-cells in worker-eggs, but as yet have been unsuccessful. Siebold has noted the same facts in eggs of wasps. 4th. Dr. Dönhoff, of Germany, in 1855, took an egg from a drone-cell, and by artificial impregnation produced a worker-bee. Such an operation, to be successful, must be performed as soon as the egg is laid.