“No--not yet--what I have done might make Pasteur sure, but I am
not at all convinced yet.... I have to get these bacilli out of
the bodies of my dying animals now ... grow them on our beef-broth
jelly, pure colonies of these microbes I must get, and cultivate
them for months, away from any living creature ... and _then_, if I
inoculate these cultivations into good healthy animals, and they get
tuberculosis ...” and Koch’s sober wrinkled face smiled for a moment.
Loeffler and Gaffky, ashamed of their jumping at conclusions, went
back awed to their own searchings.
Testing every possible combination that his head could invent, Koch
set out to try to grow his bacilli pure on beef-broth jelly. He made
a dozen different kinds of good soup for them, he kept his tubes and
bottles at the temperature of the room and the temperature of a man’s
body and the temperature of fever. He cleverly used the sick lungs
of guinea-pigs that teemed with bacilli, lungs that held no other
stray microbes which might over-grow and choke out those delicate
germs which he was sure must be the authors of consumption. The stuff
from these lungs he planted dangerously into hundreds of tubes and
bottles, but all this work ended in--nothing. In brief, those slim
bacilli that grew like weeds in tropic gardens in the bodies of his
sick animals, those microbes that swarmed in millions in sick men,
those bacilli turned up their noses--that is, they would have if they
had been equipped with noses--at the good soups and jellies that Koch
cooked for them. It was no go!
But one day a reason for his failures popped into Koch’s head: “The
trouble is that these tubercle bacilli will only grow in the bodies
of living creatures--they are maybe almost _complete_ parasites--I
must fix a food for them that is as near as possible like the stuff a
living animal’s body is made of!”
So it was that Koch invented his famous food--blood-serum jelly--for
microbes that are too finicky to grow on common provender. He went
to string-butchers and got the clear straw-colored serum from the
clotted blood of freshly slaughtered healthy cattle and carefully
heated this fluid to kill all the stray microbes that might have
fallen into it. Delicately he poured this serum into each one of
dozens of narrow test-tubes, and placed these on a slant so that
there would be a long flat surface on which to smear the sick
consumptive tissues. Then ingeniously he heated each tube just hot
enough to make the serum set, on a slant, into a clear beautiful
jelly.
Public-domain text, read in full here on John Shaqi.
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