Nitrogen Bacteria and Legumes: With special reference to red clover, cowpeas, soy beans, alfalfa, and sweet clover, on Illinois soilsHopkins, Cyril G. (Cyril George)
Science
Nitrogen Bacteria and Legumes: With special reference to red clover, cowpeas, soy beans, alfalfa, and sweet clover, on Illinois soils
Plate 5 clearly shows the results obtained and certainly furnishes
conclusive proof that the same effect is produced upon the growth of the
alfalfa whether the nitrogen-gathering bacteria used for the inoculation
are obtained from alfalfa soil, from alfalfa tubercles, from sweet clover
soil, or from sweet clover tubercles. It also illustrates the importance
of bacteria in growing alfalfa as will be seen by comparing the four
inoculated pots with the uninoculated pot, which is No. 1, on the left in
each series of views. The upper view was taken when the alfalfa plants
were five weeks old; the next series when they were six weeks old; the
next, seven weeks old; and the lower series when they were eight weeks
old, from the time of seeding.
A duplicate series of pots prepared in exactly the same manner gave
similar results.
The infected alfalfa soil was obtained from a field of three-year-old
alfalfa, which was inoculated when first seeded, with infected alfalfa
soil obtained from an old alfalfa field in Kansas. About one pound of
this soil was shaken in a quart of water, the soil allowed to settle, and
some of the nearly clear solution used for the inoculation of Pot No. 2.
The alfalfa tubercles from which bacteria were obtained were carefully
washed in distilled water to free them from adhering soil particles, and
then rubbed up in distilled water, a small amount of this water being
then used for the inoculation of Pot No. 3. The infected sweet clover
soil was obtained from a place by the roadside where sweet clover was
growing luxuriantly and well provided with root tubercles. This place was
about two miles from the nearest field ever seeded to alfalfa, so far as
known. A water extract from this soil was used to inoculate Pot No. 4.
The bacteria from sweet clover tubercles were obtained in the same manner
as those from alfalfa tubercles, and were used to inoculate Pot No. 5.
[Illustration: PLATE 5. ALFALFA: EFFECT OF BACTERIA FROM ALFALFA AND FROM
SWEET CLOVER.
Pot 1.—No bacteria.
Pots 2 and 3.—Bacteria from alfalfa.
Pots 4 and 5.—Bacteria from sweet clover.
The four series of photographs were taken five, six, seven and eight
weeks from time of planting, respectively.]
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